Faute de temps nous n'avons pas pu faire (dans l'immédiat) une analyse quantitative et nous nous sommes limités à une analyse qualitative ,
, S'agissant de premiers tests de faisabilité, nous avons préparé uniquement trois échantillons différents contenant des levures de boulanger par dilutions successives d'un premier échantillon de concentration C3 (C1 < C2 < C3) dans une solution tampon de 1413 µS/cm. Les concentrations n'ont donc pas étés déterminée avec précision, Nous pouvons Bibliographie
Towards lab-on-a-chip diagnostics for malaria elimination, Lab Chip, vol.18, issue.1, pp.75-94, 2017. ,
Linear relationship between cytoplasm resistance and hemoglobin in red blood cell hemolysis by electrical impedance spectroscopy & eight-parameter equivalent circuit, Biosens. Bioelectron, vol.119, pp.103-109, 2018. ,
Methods for the detection and identification of pathogenic bacteria: Past, present, and future, Chem. Soc. Rev, vol.46, issue.16, pp.4818-4832, 2017. ,
A label-free impedance immunosensor using screen-printed interdigitated electrodes and magnetic nanobeads for the detection of E. coli O157:H7, Biosensors, vol.5, issue.4, pp.791-803, 2015. ,
An electrorotation technique for measuring the dielectric properties of cells with simultaneous use of negative quadrupolar dielectrophoresis and electrorotation, Analyst, vol.138, issue.5, pp.1529-1537, 2013. ,
An electrical model optimization for single cell flow impedance spectroscopy, Int. J. Smart Sens. Intell. Syst, vol.9, issue.2, pp.526-536, 2016. ,
Electrical impedance microflow cytometry with oxygen control for detection of sickle cells, Sensors Actuators, B Chem, vol.255, pp.2392-2398, 2018. ,
Biosensors and Bioelectronics Multiplex immunoassays using virus-tethered gold microspheres by DC impedancebased fl ow cytometry, Biosens. Bioelectron, vol.102, pp.121-128, 2017. ,
Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity, Biosens. Bioelectron, vol.57, pp.245-253, 2014. ,
Electrical Impedance Spectroscopy for Microtissue Spheroid Analysis in Hanging-Drop Networks, ACS Sensors, vol.1, issue.8, pp.1028-1035, 2016. ,
Detection and characterization of biological cells by impedance spectroscopy, Proc. Int. Conf. Sens. Technol. ICST, pp.309-314, 2018. ,
Electrochemical immunosensors: Critical survey of different architectures and transduction strategies, TrAC -Trends Anal. Chem, vol.79, pp.88-105, 2016. ,
A microfluidic system for cell type classification based on cellular sizeindependent electrical properties, Lab Chip, vol.13, issue.12, pp.2272-2277, 2013. ,
A biosensor capable of identifying low quantities of breast cancer cells by electrical impedance spectroscopy, Sci. Rep, vol.9, issue.1, pp.1-12, 2019. ,
Impedance-Based Detection of Bacteria, Chem. Rev, vol.119, issue.1, pp.700-726, 2019. ,
Electric impedance microflow cytometry for characterization of cell disease states, Lab Chip, vol.13, issue.19, pp.3903-3909, 2013. ,
Cellular impedance biosensors for drug screening and toxin detection, Analyst, vol.132, issue.9, pp.835-841, 2007. ,
Correction of electrode polarization contributions to the dielectric properties of normal and cancerous breast tissues at audio/radiofrequencies, Phys. Med. Biol, vol.52, issue.22, pp.6589-6604, 2007. ,
SAM composition and electrode roughness affect performance of a DNA biosensor for antibiotic resistance, Biosensors, vol.9, issue.1, 2019. ,
Single cell dielectric spectroscopy, J. Phys. D. Appl. Phys, vol.40, issue.1, pp.61-70, 2007. ,
Interdigitated array microelectrode based impedance biosensor coupled with magnetic nanoparticle-antibody conjugates for detection of Escherichia coli O157:H7 in food samples, Biosens. Bioelectron, vol.22, issue.11, pp.2408-2414, 2007. ,
All electronic approach for high-throughput cell trapping and lysis with electrical impedance monitoring, Biosens. Bioelectron, vol.54, pp.462-467, 2014. ,
Impedance spectroscopy flow cytometry: Onchip label-free cell differentiation, Cytom. Part A, vol.65, issue.2, pp.124-132, 2005. ,
Dielectric properties of isolated adrenal chromaffin cells determined by microfluidic impedance spectroscopy, Bioelectrochemistry, vol.119, pp.84-91, 2018. ,
, Biomedical Sensors and Measurement, 2011.
Design rule for optimization of microelectrodes used in electric cell-substrate impedance sensing (ECIS), Biosens. Bioelectron, vol.24, issue.7, pp.2071-2076, 2009. ,
, , p.3, 2014.
Cohésion et dissolution des solides ioniques et moléculaires, p.9, 2019. ,
The Electric Resistance and Capacity of Blood for Frequencies Between 800 and 4(1/2) Million Cycles, J. Gen. Physiol, vol.9, issue.2, pp.153-67, 1925. ,
Caractérisation diélectrique du sang par spectroscopie de bio-impédance dans la bande [1 MHz -1GHZ]: conception et réalisation d'une cellule de mesure, 2011. ,
Polar Molecules, 1929. ,
Dispersion and absorption in dielectrics I. Alternating current characteristics, J. Chem. Phys, 1941. ,
Electrical Properties of Tissue and Cell Suspensions, Advances in biological and medical physics, pp.147-209, 1957. ,
Impedance spectroscopy on living tissue for determination of the state of organs, Bioelectrochemistry Bioenerg, vol.45, issue.2, pp.145-149, 1998. ,
, Impedance Measurement Handbook, A guide to measurement technology and techniques, 6th Edition -Application Note, pp.5-7
Lab-on-chip technology: A review on design trends and future scope in biomedical applications, Int. J. Bio-Science Bio-Technology, vol.8, issue.5, pp.311-322, 2016. ,
Microfluidics towards single cell resolution protein analysis, TrAC Trends Anal. Chem, 2019. ,
, Microhydrodynamique et fluides complexes, 2011.
Lab on a chip for continuous-flow magnetic cell separation, Lab Chip, vol.15, issue.4, pp.959-970, 2015. ,
Red blood cell quantification microfluidic chip using polyelectrolytic gel electrodes, Electrophoresis, vol.30, issue.9, pp.1464-1469, 2009. ,
, Blood Pressure Measurement at Home, vol.14, pp.3453-3456
Design and Simulation of MEMS Capacitive Pressure Sensor Array for Wide Range Pressure Measurement, Int. J. Comput. Appl, vol.163, issue.6, pp.39-46, 2017. ,
Capacitive sensing for non-invasive breathing and heart monitoring in non-restrained, non-sedated laboratory mice, Sensors (Switzerland), vol.16, issue.7, 2016. ,
Solid state electrochemical gas sensor for the quantitative determination of carbon dioxide, Sensors Actuators, B Chem, vol.265, pp.27-34, 2018. ,
Miniaturized planar room temperature ionic liquid electrochemical gas sensor for rapid multiple gas pollutants monitoring, Sensors Actuators, B Chem, vol.255, pp.638-646, 2018. ,
Biosensors, 2019. ,
Peptide nanotube-modified electrodes for enzyme-biosensor applications, Anal. Chem, vol.77, issue.16, pp.5155-5159, 2005. ,
Enzyme based amperometric biosensors, Curr. Opin. Electrochem, vol.10, pp.157-173, 2018. ,
A micromachined impedance biosensor for accurate and rapid detection of E. Coli O157:H7, RSC Adv, vol.3, issue.48, pp.26297-26306, 2013. ,
High-throughput impedance spectroscopy biosensor array chip Subject Areas : Author for correspondence, Philos. Trans. R. Soc, 2014. ,
A CMOS electrochemical impedance spectroscopy (EIS) biosensor array, IEEE Trans. Biomed. Circuits Syst, vol.4, issue.6, pp.379-390, 2010. ,
, Biomedical Nanosensors, 2018.
Whole cell biosensors, Sensors Actuators, B Chem, vol.34, issue.1-3, pp.270-275, 1996. ,
Recent Advances in Application of Biosensors in Tissue Engineering, Biomed Res. Int, vol.2014, pp.1-18, 2014. ,
Cell-based biosensors: Recent trends, challenges and future perspectives, Biosens. Bioelectron, vol.141, p.111435, 2019. ,
A Comprehensive Model for Whole Cell Sensing and Transmembrane Potential Measurement Using FET Biosensors, ECS J. Solid State Sci. Technol, vol.7, issue.7, pp.3001-3008, 2018. ,
Dual functional extracellular recording using a light-addressable potentiometric sensor for bitter signal transduction, Anal. Chim. Acta, vol.1022, pp.106-112, 2018. ,
Biosensors based on the energy metabolism of living cells: The physical chemistry and cell biology of extracellular acidification, Biosens. Bioelectron, vol.7, issue.4, pp.255-272, 1992. ,
Plug-and-play metabolic transducers expand the chemical detection space of cell-free biosensors, Nat. Commun, vol.10, issue.1, pp.1-8, 2019. ,
URL : https://hal.archives-ouvertes.fr/hal-02399479
All electronic approach for high-throughput cell trapping and lysis with electrical impedance monitoring, Biosens. Bioelectron, vol.54, pp.462-467, 2014. ,
Conception et réalisation d'une sonde de mesure des caractéristiques électromagnétiques des tissus biologiques et de l'instrumentation associée : spectroscopie de bioimpédance par la méthode V/I dans la bande 1MHz, 1998. ,
Conception et réalisation d'une cellule de caractérisation des tissus biologiques par spectroscopie de bioimpédance dans la gamme fréquentielle, 100 Hz-1MHz : application aux tissus osseux, prise en compte de l'anisotropie, 2000. ,
Spectroscopie d'impédance appliquée à la composition corporelle en néphrologie et en dialyse : caractérisation des facteurs d'influence, 2013. ,
Bacteria detection and differentiation using impedance flow cytometry, Sensors (Switzerland), vol.18, issue.10, pp.1-12, 2018. ,
Microfabricated Multi-Frequency Particle Impedance Characterization System, Micro Total Anal. Syst, pp.265-268, 2000. ,
Dielectric spectroscopy in a micromachined flow cytometer: Theoretical and practical considerations, Lab Chip, vol.4, issue.3, pp.241-251, 2004. ,
A label-free, microfluidics and interdigitated array microelectrode-based impedance biosensor in combination with nanoparticles immunoseparation for detection of Escherichia coli O157:H7 in food samples, Sensors Actuators, B Chem, vol.128, issue.1, pp.99-107, 2007. ,
Electrical impedance spectroscopy for detection of bacterial cells in suspensions using interdigitated microelectrodes, Talanta, vol.74, issue.5, pp.1621-1629, 2008. ,
Specific and targeted detection of viable Escherichia coli O157:H7 using a sensitive and reusable impedance biosensor with dose and time response studies, Talanta, vol.94, pp.84-89, 2012. ,
Concept for E.coli detection using interdigitated microelectrode impedance sensor, Proc. Annu. Int. Conf. IEEE Eng. Med. Biol. Soc. EMBS, pp.1712-1715, 2013. ,
On-line control of cellular adhesion with impedance measurements using interdigitated electrode structures, Med. Biol. Eng. Comput, vol.36, issue.3, pp.365-370, 1998. ,
Design and Realization of a Planar Interdigital Microsensor for Biological Medium Characterization, Next Generation Sensors and Systems, vol.16, pp.1-330, 2015. ,
URL : https://hal.archives-ouvertes.fr/hal-01293432
High-speed particle detection in a micro-Coulter counter with twodimensional adjustable aperture, Biosens. Bioelectron, vol.24, issue.2, pp.290-296, 2008. ,
Microfluidic biosensor for single cell high speed flow impedance spectroscopy, 8Th Conf. Sens. Technol, vol.7198, pp.343-347, 2014. ,
Spectroscopie d'impédance électrique par biocapteur à micro-électrodes : application à la cytométrie de flux de cellules sanguines, 2013. ,
Contribution à la mesure de bioimpédance électrique de cellules biologiques par micro-capteurs interdigités: optimisation, conception et validation de capteurs ,
Mesure de bioimpédance électrique par capteurs interdigités, 2012. ,
Étude et conception d'une plateforme microfluidique pour la détection de séquence ADN par spectroscopie d'impédance ,
Cell Based Biosensors Using, 1998. ,
Impedance studies of bio-behavior and chemosensitivity of cancer cells by micro-electrode arrays, Biosens. Bioelectron, vol.24, issue.5, pp.1305-1310, 2009. ,
Impedance spectroscopy: Over 35 years of electrochemical sensor optimization, Electrochim. Acta, vol.51, issue.28, pp.6217-6229, 2006. ,
Use of an Insulation Layer on the Connection Tracks of a Biosensor with Coplanar Electrodes to Increase the Normalized Impedance Variation, 2019. ,
Coplanar capacitance sensors for detecting water intrusion in composite structures, Meas. Sci. Technol, vol.19, issue.7, 2008. ,
Dielectrophoresis for bioparticle manipulation, Int. J. Mol. Sci, vol.15, issue.10, pp.18281-18309, 2014. ,
Dielectrophoretic characterization of cells in a stationary nanoliter droplet array with generated chemical gradients, Biomed. Microdevices, vol.17, issue.5, pp.1-10, 2015. ,
Magnetic Nanoparticle-Antibody Conjugates for the Separation of Escherichia coli O157:H7 in Ground Beef, J. Food Prot, vol.68, issue.9, pp.1804-1811, 2016. ,
PDMS bonding by means of a portable, low-cost corona system, Lab Chip, vol.6, issue.12, pp.1548-1549, 2006. ,
, Guilhem Velvé Casquillas
PDMS: A REVIEW ,
E4990A Impedance Analyzer, 2016. ,
, Liste des publications et communications Articles de revues
Use of an Insulation Layer on the Connection Tracks of a Biosensor with Coplanar Electrodes to Increasethe Normalized Impedance Variation, Biosensors, vol.9, p.108, 2019. ,
Influence of Electrode Connection Tracks on Biological Cell Measurements by Impedance, Sensors, vol.19, issue.13, p.2839, 2019. ,
Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance, Sensors, vol.19, issue.15, p.3366, 2019. ,
Detection and characterization of biological cells by impedance spectroscopy, 12th International Conference on Sensing Technology, 2018. ,
Increasing operating frequency band of cytometric biosensor for single cell impedance characterization, 12th International Conference on Sensing Technology, 2018. ,
Caractérisation de cellules biologiques par spectroscopie d'impédance, Coloque GDR SOC, 2018. ,
Influence du routage des pistes de connexions sur la sensibilité de biocapteurs à matrice d'électrodes carrées ,
High reliability microfluidic biosensor for single cell impedance cytometry, Coloque GDR SOC SIP 2017 ,