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, Methods: Anesthetized and ventilated rats (n=44) were subjected to an endotoxemic shock following lipopolysaccharide (LPS) infusion. CµPO2 was measured in exposed kidneys using a phosphorescence-quenching method. Rats were randomly assigned to 5 groups: (1) unresuscitated LPS, (2) ringer's acetate (LPS+RA), PO2 (CµPO2) and skeletal microcirculatory flow thereby limiting sepsis-induced acute kidney injury (AKI) in a rat model of endotoxemia

, ) LPS+RA+PEGHbCO (320mg.kg -1 )

, and myeloperoxidase-stained leukocytes in glomerulus and peritubular areas were analyzed. Sepsis-induced histological damages were assessed. Finally, plasma levels of IL-6, heme oxygenase-1, malondialdehyde, and syndecan-1 were assessed by ELISA. Results: CµPO2 was higher in groups resuscitated with PEGHbCO, 36±6 mmHg (mean diff, p.95

, PEGHbCO, reduced the number of non-flowing, intermittent or sluggish capillaries compared to resuscitation with RA alone (P<0.05) while increasing the number of normally perfused vessels (P<0.05). Sepsis-induced kidney immunohistochemistry and histological alterations were not mitigated by PEGHbCO 1h after resuscitation. Renal leukocyte infiltration and plasma levels of IL-6, heme oxygenase-1, malondialdehyde, and syndecan-1 were similar across groups, P=0.023), for LPS+RA+PEGHbCO and LPS+RA+PEGHbCO+NE respectively, compared to 23±12mmHg for LPS+RA

, PEGylated carboxyhemoglobin, carbon monoxide, hemoglobin-based oxygen carrier, renal injury, sepsis Chapter 11 excluded any interference between Palladium(II)-meso-tetra(4-carboxyphenyl)-porphyrin (Pd-TCCP) and PEGHbCO (in vitro) prior to the in vivo experiment. Kidney function Plasma creatinine and urine creatinine were measured using an automatic analyzer, Keywords: acute kidney injury, p.702

R. Diagnostics and R. Diagnostics, We established a calibration curve for creatinine levels prior to measurements to correct for a significant interference due to the presence of PEGylated-carboxyhemoglobin. The glomerular filtration rate was estimated at the end of the experiment using the measurement of creatinine clearance (Clcrea): Clcrea=(UCrea x V)/(time x PCrea) where UCrea is the concentration of creatinine in urine (mmol.l-1), V is the urine volume (ml) per unit time of urine collection and PCrea is the concentration of creatinine in plasma (mmol.l-1). The renal oxygen delivery (DO2) and consumption (VO2hand-held in vivo microscope using incident dark-field (IDF) imaging, CytoCam? (Braedius Scientific

, Central Venous Pressure, NE: norepinephrine. Repeated measures 2-way ANOVA test used with Bonferroni's correction to adjust for multiple comparisons. * adjusted P<0.01 vs, MAP: Mean Arterial Pressure, RBF: Renal Blood Flow

L. Lps+ra+peghbco, LPS+RA+PEGHbCO+NE groups but not in the LPS+RA group

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, PEGylated carboxyhemoglobin resuscitation in endotoxemia of O2 (DO2) (ml O2.min -1 ) was determined at the end of the experiment as follows: Renal DO2=

R. Cao2-x, Venous oxygen content (CvO2) was determined by analysis of a blood gas of the renal vein, Renal oxygen consumption (VO2ren, in ml O2.min -1 ) was determined as follows: VO2ren = C(a

R. O2-x, The renal energy efficiency for sodium transport (VO2ren/TNa + ) was assessed using the ratio of the total amount of VO2ren over the total amount of sodium reabsorbed (TNa + , millimolar per minute)

, Kidneys were harvested, fixed in 4% formalin and embedded in paraffin. For immunohistochemical analysis, kidney sections (5 µm) were deparaffinized with xylene and rehydrated with decreasing percentages of ethanol and finally with water. Antigen retrieval was accomplished by microwaving slides in citrate buffer, p.9003

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, Smartphone to-do list application to improve workflow in an intensive care unit: A superiority quasi-experimental study, Int J Med Inform, vol.136, p.104085, 2020.

P. Guerci, H. Bellut, M. Mokhtari, J. Gaudefroy, N. Mongardon et al., Outcomes of Stenotrophomonas maltophilia hospital-acquired pneumonia in intensive care unit: a nationwide retrospective study, Crit Care, vol.23, issue.1, p.371, 2019.
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L. Leon, P. Guerci, E. Pape, N. Thilly, A. Luc et al., Serum and peritoneal exudate concentrations after high doses of ?-lactams in critically ill patients with severe intra-abdominal infections: an observational prospective study, J Antimicrob Chemother, p.407, 2019.
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T. Belveyre, P. Guerci, E. Pape, N. Thilly, K. Hosseini et al., Antibiotic prophylaxis with high-dose cefoxitin in bariatric surgery: an observational prospective single center study
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, Antimicrob Agents Chemother, 2019.

P. Guerci, B. Ergin, A. Kapucu, M. P. Hilty, R. Jubin et al., Effect of Polyethyleneglycolated Carboxyhemoglobin on Renal Microcirculation in a Rat Model of Hemorrhagic Shock, Anesthesiology, vol.131, issue.5, pp.1110-1124, 2019.

S. Rimbert, D. Schmartz, L. Bougrain, C. Meistelman, C. Baumann et al., MOTANA: study protocol to investigate motor cerebral activity during a propofol sedation, Trials, vol.20, issue.1, p.534, 2019.
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N. Mongardon, A. Bouglé, C. Sola, G. Bouroche, F. Verdonk et al.,

, Publication outcome of abstracts presented at an Anesthesiology and Critical Care Medicine meeting: Does being a junior presenter matter?, J Clin Anesth, vol.60, pp.49-50, 2019.

M. P. Hilty, P. Guerci, Y. Ince, F. Toraman, and C. Ince, MicroTools enables automated quantification of capillary density and red blood cell velocity in handheld vital microscopy, Commun Biol, vol.2, p.217, 2019.

P. Guerci, B. Ergin, and C. Ince, Response, vol.129, pp.102-103, 2019.
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M. Garnier, S. Gallah, S. Vimont, Y. Benzerara, V. Labbe et al., Multicentre randomised controlled trial to investigate usefulness of the rapid diagnostic ?LACTA test performed directly on bacterial cell pellets from respiratory, urinary or blood samples for the early de-escalation of carbapenems in septic intensive care unit patients: the BLUE-CarbA protocol, BMJ Open, vol.9, issue.2, p.24561, 2019.

Z. Uz, T. M. Van-gulik, M. D. Aydemirli, P. Guerci, Y. Ince et al., Identification and quantification of human microcirculatory leukocytes using handheld video microscopes at the bedside, J Appl Physiol, vol.124, issue.6, pp.1550-1557, 2018.

Z. Uz, C. Ince, P. Guerci, Y. Ince, R. Araujo et al., Recruitment of sublingual microcirculation using handheld incident dark field imaging as a routine measurement tool during the postoperative de-escalation phase-a pilot study in post ICU cardiac surgery patients, Perioper Med (Lond), vol.7, p.18, 2018.

E. Novy, F. Laithier, M. Machouart, E. Albuisson, P. Guerci et al., Determination of 1,3-?-D-glucan in the peritoneal fluid for the diagnosis of intra-abdominal candidiasis in critically ill patients: a pilot study, Minerva Anestesiol, vol.84, issue.12, pp.1369-1376, 2018.

M. Jabaudon, N. Belhadj-tahar, T. Rimmelé, J. -. Boyau, O. Bulyez et al.,

Y. Malledant, M. Leone, P. Abback, F. Tamion, H. Dupont et al., Thoracic Epidural Analgesia and Mortality in Acute Pancreatitis: A Multicenter Propensity Analysis, Crit Care Med, vol.46, issue.3, pp.198-205, 2018.
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P. Guerci, B. Ergin, Z. Uz, Y. Ince, M. Westphal et al., Glycocalyx Degradation Is Independent of Vascular Barrier Permeability Increase in Nontraumatic Hemorrhagic Shock in Rats, Anesth Analg, vol.129, issue.2, pp.598-607, 2019.

P. Guerci, J. Claudot, E. Novy, N. Settembre, J. Lalot et al., Immediate postoperative plasma neutrophil gelatinase-associated lipocalin to predict acute kidney injury after major open abdominal aortic surgery: A prospective observational, Anaesth Crit Care Pain Med, vol.37, issue.4, pp.327-334, 2018.

M. Bonnassieux, A. Duclos, A. G. Schneider, A. Schmidt, S. Bénard et al., Renal Replacement Therapy Modality in the ICU and Renal Recovery at Hospital Discharge
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, Crit Care Med, vol.46, issue.2, pp.102-110, 2018.

F. Vial, N. Hime, J. Feugeas, N. Thilly, P. Guerci et al., Ultrasound assessment of gastric content in the immediate postpartum period: a prospective observational descriptive study, Acta Anaesthesiol Scand, vol.61, issue.7, pp.730-739, 2017.

P. Guerci, Y. Ince, P. Heeman, D. Faber, B. Ergin et al., A LED-based phosphorimeter for measurement of microcirculatory oxygen pressure, J Appl Physiol, vol.122, issue.2, pp.307-316, 2017.

P. Guerci, B. Ergin, and C. Ince, The macro-and microcirculation of the kidney, Best Pract Res Clin Anaesthesiol, vol.31, issue.3, pp.315-329, 2017.

S. Bulyez, B. Pereira, E. Caumon, E. Imhoff, L. Roszyk et al.,

E. Escudier, A. Sossou, D. Morand, V. Sapin, J. Constantin et al., Epidural analgesia in critically ill patients with acute pancreatitis: the multicentre randomised controlled EPIPAN study protocol, BMJ Open, vol.7, issue.5, p.15280, 2017.
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N. E. Baka, F. Vial, G. Iohom, P. Guerci, C. Hubert et al., The effect of nefopam on lactation after caesarean section: a single-blind randomised trial, Int J Obstet Anesth, vol.31, pp.84-90, 2017.

L. Avercenc-léger, P. Guerci, J. Virion, G. Cauchois, S. Hupont et al., Umbilical cord-derived mesenchymal stromal cells: predictive obstetric factors for cell proliferation and chondrogenic differentiation, Stem Cell Res Ther, vol.8, issue.1, p.161, 2017.

M. Legrand, L. Cam, B. Perbet, S. Roger, C. Darmon et al., Urine sodium concentration to predict fluid responsiveness in oliguric ICU patients: a prospective multicenter observational study, Crit Care, vol.20, issue.1, p.165, 2016.
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C. Ince and P. Guerci, Why and when the microcirculation becomes disassociated from the macrocirculation, Intensive Care Med, vol.42, issue.10, pp.1645-1646, 2016.

B. Ergin, P. Guerci, L. Zafrani, F. Nocken, A. Kandil et al., Effects of N-acetylcysteine (NAC) supplementation in resuscitation fluids on renal microcirculatory oxygenation, inflammation, and function in a rat model of endotoxemia, Intensive Care Med Exp, vol.4, issue.1, p.29, 2016.

B. Ergin, A. Kapucu, P. Guerci, and C. Ince, The role of bicarbonate precursors in balanced fluids during haemorrhagic shock with and without compromised liver function, Br J Anaesth, vol.117, issue.4, pp.521-528, 2016.

F. Vial, S. Mory, P. Guerci, B. Grandjean, L. Petry et al.,

, Can J Anaesth, vol.62, issue.6, pp.627-633, 2015.

J. Raft, P. Guerci, V. Harter, T. Fuchs-buder, and C. Meistelman, Biological evaluation of the effect of sugammadex on hemostasis and bleeding, Korean J Anesthesiol, vol.68, issue.1, pp.17-21, 2015.

M. Poussel, P. Guerci, P. Kaminsky, M. Heymonet, N. Roux-buisson et al., Exertional Heat Stroke and Susceptibility to Malignant Hyperthermia in an Athlete: Evidence for a Link?, J Athl Train, vol.50, issue.11, pp.1212-1214, 2015.
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P. Guerci, F. Vial, J. Feugeas, M. Pop, N. Baka et al., Cerebral oximetry assessed by near-infrared spectrometry during preeclampsia: an observational study: impact of magnesium sulfate administration, Crit Care Med, vol.42, issue.11, pp.2379-2386, 2014.

P. Guerci, N. Tran, P. Menu, M. Losser, C. Meistelman et al., Impact of fluid resuscitation with hypertonic-hydroxyethyl starch versus lactated ringer on hemorheology and microcirculation in hemorrhagic shock, Clin Hemorheol Microcirc, vol.56, issue.4, pp.301-317, 2014.
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P. Guerci, F. Vial, U. Mcnelis, M. R. Losser, J. Raft et al., Neuraxial anesthesia in patients with intracranial hypertension or cerebrospinal fluid shunting systems: what should the anesthetist know, Minerva Anestesiol, vol.80, issue.9, pp.1030-1045, 2014.

B. Christ, P. Guerci, C. Baumann, C. Meistelman, and D. Schmartz, Influence of neuromuscular block and reversal on bispectral index and NeuroSense values, Eur J Anaesthesiol, vol.31, issue.8, pp.437-439, 2014.

O. Breton, F. Vial, J. Feugeas, K. Podrez, K. Hosseini et al., Les membres du bureau de l'Institut lorrain d'anesthésie-réanimation (Ilar)

, Ann Fr Anesth Reanim, vol.33, issue.11, pp.581-586, 2014.

D. Schmartz, P. Guerci, and T. Fuchs-buder,

, Anesthesiology, vol.118, issue.3, p.754, 2013.

E. Novy, C. Charpentier, P. Guerci, N. Settembre, S. Malikov et al.,

, Ann Fr Anesth Reanim, vol.32, issue.11, pp.799-802, 2013.

N. Karangwa, J. Vial, F. Uwambazimana, J. Guerci, P. Ndoli et al., Postoperative pain management after caesarean section in Kigali University Medical Center

, Ann Fr Anesth Reanim, vol.32, issue.9, pp.631-632, 2013.

A. Kimmoun, P. Guerci, C. Bridey, N. Ducrocq, F. Vanhuyse et al., Prone positioning use to hasten veno-venous ECMO weaning in ARDS, Intensive Care Med, vol.39, issue.10, pp.1877-1879, 2013.

P. Guerci, E. Novy, F. Vial, B. Lecointe, M. Geffroy-bellan et al.,

, Sulprostone for postpartum hemorrhage in a parturient with a history of Tako-tsubo cardiomyopathy, J Clin Anesth, vol.25, issue.4, pp.327-330, 2013.

P. Guerci, F. Vial, J. Raft, U. M. Nelis, S. Mory et al.,

, Ann Fr Anesth Reanim, vol.32, issue.2, pp.115-117, 2013.

P. Guerci, E. Novy, J. Guibert, F. Vial, J. Malinovsky et al.,

, Ann Fr Anesth Reanim, vol.32, issue.5, pp.364-367, 2013.

B. Grandjean, P. Guerci, J. Raft, T. Fuchs-buder, and H. Bouaziz,

, Ann Fr Anesth Reanim, vol.32, issue.5, pp.378-379, 2013.

T. Fuchs-buder, C. Baumann, D. Guis, J. Guerci, P. Meistelman et al., Low-dose neostigmine to antagonise shallow atracurium neuromuscular block during inhalational anaesthesia: A randomised controlled trial, Eur J Anaesthesiol, vol.30, issue.10, pp.594-598, 2013.

V. Jacques, P. Guerci, F. Vial, F. Abel, and H. Bouaziz, Dissection of the descending aorta complicated by preeclampsia at 30 weeks of gestation: medical treatment and caesarean delivery

, Ann Fr Anesth Reanim, vol.31, issue.1, pp.67-71, 2012.

N. Hime, T. Auchet, P. Guerci, F. Vial, U. Mcnelis et al., Vaginal delivery in a parturient excessively anticoagulated with fondaparinux, Int J Obstet Anesth, vol.21, issue.4, pp.385-387, 2012.

P. Guerci, F. Vial, and H. Bouaziz,

, Can J Anaesth, vol.59, issue.1, pp.114-115, 2012.

P. Guerci, F. Vial, J. Raft, C. Meistelman, and H. Bouaziz,

, Ann Fr Anesth Reanim, vol.31, issue.7-8, pp.632-634, 2012.

P. Guerci, F. Vial, J. Raft, G. Jurkolow, and H. Bouaziz,

, Ann Fr Anesth Reanim, vol.31, issue.5, pp.485-486, 2012.