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, L'huile des graines de M. charantia a été extraite par extraction enzymatique aqueuse (AEE) et le rendement en huile a augmenté avec la condition optimale pour extraire l'huile de graines était d'utiliser HEL1:X7 (5:1.25). L'huile de graines ainsi que la teneur en lignine, hémicelullose, sucres solubles et acides uroniques étaient différentes selon le type de cocktail enzymatique. D'autre part, l'extrait d'acétate d'éthyle de la chair du fruit de M. charantia a inhibé 38.11±1.06 % de la DENV-2 protéase NS2B/NS3. Sur la base de l'analyse GC-MS, on a trouvé la trace d'acide gallique et on a sélectionné les cadres structurels de base pour la synthèse de l'inhibiteur de protéase DENV-2 NS2B/NS3. Les dérivés de l'indanone substituée par le pyrazolyle, 90a-t, ont été synthétisés et caractérisés par analyse FTIR, RMN et LC-MS. Un pourcentage approprié de rendement des composés a été obtenu. Les composés 90a-t ont été soumis à une étude in silico en utilisant AutoDock4.2 pour identifier le mode de liaison et la conformation avec la structure cristalline de la protéine d'homologie de Wichapong. Cinq composés ont obtenu une énergie libre de liaison (FEB) inférieure à -7.55 kcal/mol. Le composé 90p a montré huit interactions de liaison hydrogène, une interaction d'empilement ?-?, et une interaction ?-alkyle avec le résidu d'acide aminé dans la DENV-2protéase NS2B/NS3. Enfin, les études in vitro des cinq principaux composés synthétisés par ancrage vers la DENV-2 protéase NS2B/NS3 en utilisant le substrat peptidique fluorogène Boc-Gly-Arg-7-amino-4-méthylcoumarine ont été menées. La panduratine A a été utilisée comme témoin positif. Le composé 90p a montré une forte activité d, Seuls des soins médicaux complémentaires et une thérapie par les fluides sont disponibles pour lutter contre la dengue. Certains patients optent pour des mesures de prévention et de traitement utilisées par les peuples anciens ou des remèdes populaires pour traiter la maladie. L'un des remèdes populaires utilisés par les patients atteints, vol.87

, On the other hand, the M. of the DENV-2 NS2B/NS3 protease. Based on the GC-MS analysis the trace of gallic acid was found and was selected the basic structural frameworks for synthesis of the DENV-2 NS2B/NS3 protease inhibitor. The pyrazolyl substituted indanone derivatives, 90a-t were synthesized and characterized using FTIR, NMR, and LC-MS analysisbond interactions, one ?-? stacking interaction, and one ?-alkyl interaction with the amino acid residue in the DENV-2 NS2B/NS3 protease, Lastly, the in vitro studies of top five docked compoundtowards DENV-2 NS2B/NS3 protease by using fluorogenic peptide substrate Boc-Gly-Arg-7-amino