Genetic instability of whiG gene during the aerial mycelium development of Streptomyces ambofaciens ATCC23877 under different conditions of nitrogen limitations - DynAMic - Dynamique des génomes et adaptation microbienne Accéder directement au contenu
Article Dans Une Revue Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis Année : 2006

Genetic instability of whiG gene during the aerial mycelium development of Streptomyces ambofaciens ATCC23877 under different conditions of nitrogen limitations

Résumé

In Streptomyces ambofaciens, white papillae that genetic instability events generate during aerial mycelium growth, give rise to Pig-pap mutants which are unable to sporulate and devoid of large genome rearrangement. Knowing that genetic and environmental factors can influence the number of papillae per colony, we investigated the effect of nutrient limitated conditions of growth on the formation of white papillae. We observed that under nitrogen limitation and, most particularly, under amino acid limitation, the number of papillae per colony dramatically increased. Most of the Pig-pap mutants deriving from such papillae displayed a mutation in the whiG gene, which encodes the sigma factor σwhiG which is absolutely required for the sporulation process. In most cases, the mutation led to a loss of function. We showed that the Pig-pap mutants deriving from papillae appearing under usual growth conditions also frequently displayed null mutation of whiG too. As the whiG mutation ratio among the Pig-pap mutants isolated with or without nitrogen limited conditions did not change, the results described in this paper suggest that the production of papillae could constitute a response of S. ambofaciens to an amino acid limitation.

Dates et versions

hal-02653652 , version 1 (29-05-2020)

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Magali Genay, Sibel Catakli, Alexandra Kleinclauss, Axelle Andrieux, Bernard Decaris, et al.. Genetic instability of whiG gene during the aerial mycelium development of Streptomyces ambofaciens ATCC23877 under different conditions of nitrogen limitations. Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2006, 595 (1-2), pp.80-90. ⟨10.1016/j.mrfmmm.2005.10.005⟩. ⟨hal-02653652⟩
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