Skip to Main content Skip to Navigation
Journal articles

Hydroxyapatite incorporated into collagen gels for mesenchymal stem cell culture

Abstract : Collagen gels could be used as carriers in tissue engineering to improve cell retention and distribution in the defect. In other respect hydroxyapatite could be added to gels to improve mechanical properties and regulate gel contraction. The aim of this work was to analyze the feasibility to incorporate hydroxyapatite into collagen gels and culture mesenchymal stem cells inside it. Human bone marrow mesenchymal stem cells (hMSC-BM) were used in this study. Gels were prepared by mixing rat tail type I collagen, hydroxyapatite microparticles and MSCs. After polymerization gels were kept in culture while gel contraction and mechanical properties were studied. In parallel, cell viability and morphology were analyzed. Gels became free-floating gels contracted from day 3, only in the presence of cells. A linear rapid contraction phase was observed until day 7, then a very slow contraction phase took place. The incorporation of hydroxyapatite improved gel stability and mechanical properties. Cells were randomly distributed on the gel and a few dead cells were observed all over the experiment. This study shows the feasibility and biocompatibility of hydroxyapatite supplemented collagen gels for the culture of mesenchymal stem cells that could be used as scaffolds for cell delivery in osteoarticular regenerative medicine.
Document type :
Journal articles
Complete list of metadata

https://hal.univ-lorraine.fr/hal-01431266
Contributor : Lemta Ul <>
Submitted on : Tuesday, January 10, 2017 - 4:32:12 PM
Last modification on : Monday, March 29, 2021 - 4:03:06 PM

Identifiers

Citation

F. Laydi, Rachid Rahouadj, G. Cauchois, Jean-François Stoltz, N. de Isla. Hydroxyapatite incorporated into collagen gels for mesenchymal stem cell culture. Bio-Medical Materials and Engineering, IOS Press, 2013, 23 (4), pp.311-315. ⟨10.3233/BME-130755⟩. ⟨hal-01431266⟩

Share

Metrics

Record views

123