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Evaluation of the Proliferative Effects Induced by Low-Level Laser Therapy in Bone Marrow Stem Cell Culture

Abstract : Objective: The objective of this study was to evaluate the effect of laser irradiation on dog bone marrow stem cells. Background data: Low doses of low-level red laser positively affect the viability of mesenchymal stem cells, and also increase proliferation. Methods: Low-level laser (wavelength, 660nm; power output, 50mW), was applied to dog bone marrow stem cell cultures (DBMSC). The energy densities delivered varied from 1 to 12J/cm(2). The effect of the laser irradiation was evaluated on cell proliferation measured with the MTT colorimetric test, cell cycle phase, and on lipidic peroxidation (free radical production). Results: The results indicate that laser irradiation to DBMSC did not change the morphology of the cells, but significantly increased their viability and the number of cells at the G2/M phase with 6, 10, and 12J/cm(2). On the other hand, malonaldehyde production was significantly enhanced with 8J/cm(2). Conclusions: The parameters used to irradiate DBMSC increased significantly proliferation without producing high levels of reactive oxygen species (ROS).
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https://hal.univ-lorraine.fr/hal-01451667
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Submitted on : Wednesday, February 1, 2017 - 12:33:14 PM
Last modification on : Wednesday, December 22, 2021 - 3:34:02 PM

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Marcos Fernando Xisto Braga Cavalcanti, Durvanei A. Maria, Natalia De Isla, Ernesto Cesar Pinto Leal-Junior, Jon Joensen, et al.. Evaluation of the Proliferative Effects Induced by Low-Level Laser Therapy in Bone Marrow Stem Cell Culture. Photomedicine and Laser Surgery, Mary Ann Liebert, 2015, 33 (12), pp.610-616. ⟨10.1089/pho.2014.3864⟩. ⟨hal-01451667⟩

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