UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis

Abstract : Introduction: The objective of this study was to investigate the possible role of UDP-glucose dehydrogenase (UGDH) in osteoarthritis (OA) and uncover whether, furthermore how interleukin-1beta (IL-1 beta) affects UGDH gene expression. Methods: UGDH specific siRNAs were applied to determine the role of UGDH in proteoglycan (PG) synthesis in human articular chondrocytes. Protein levels of UGDH and Sp1 in human and rat OA cartilage were detected. Then, human primary chondrocytes were treated with IL-1 beta to find out whether and how IL-1 beta could regulate the gene expression of UGDH and its trans-regulators, that is Sp1, Sp3 and c-Krox. Finally, p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580 and stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) inhibitor SP600125 were used to pick out the pathway that mediated the IL-1 beta-modulated PGs synthesis and gene expression of UGDH, Sp1, Sp3 and c-Krox. Results: UGDH specific siRNAs markedly inhibited UGDH mRNA and protein expression, and thus led to an obvious suppression of PGs synthesis in human articular chondrocytes. UGDH protein level in human and rat OA cartilage were much lower than the corresponding controls and negatively correlated to the degree of OA. Decrease in Sp1 protein level was also observed in human and rat OA cartilage respectively. Meanwhile, IL-1 beta suppressed UGDH gene expression in human articular chondrocytes in the late phase, which also modulated gene expression of Sp1, Sp3 and c-Krox and increased both Sp3/Sp1 and c-Krox/Sp1 ratio. Moreover, the inhibition of SAP/JNK and p38 MAPK pathways both resulted in an obvious attenuation of the IL-1 beta-induced suppression on the UGDH gene expression. Conclusions: UGDH is essential in the PGs synthesis of articular chondrocytes, while the suppressed expression of UGDH might probably be involved in advanced OA, partly due to the modulation of p38 MAPK and SAP/JNK pathways and its trans-regulators by IL-1 beta.
Type de document :
Article dans une revue
Arthritis Research and Therapy, BioMed Central, 2014, 16 (6), 〈10.1186/s13075-014-0484-2〉
Liste complète des métadonnées

https://hal.univ-lorraine.fr/hal-01453199
Contributeur : Imopa Ul <>
Soumis le : jeudi 2 février 2017 - 16:12:26
Dernière modification le : mercredi 16 mai 2018 - 11:48:04

Lien texte intégral

Identifiants

Citation

Yinxian Wen, Jing Li, Linlong Wang, Kai Tie, Jacques Magdalou, et al.. UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis. Arthritis Research and Therapy, BioMed Central, 2014, 16 (6), 〈10.1186/s13075-014-0484-2〉. 〈hal-01453199〉

Partager

Métriques

Consultations de la notice

108