Antigen I/II encoded by integrative and conjugative elements of Streptococcus agalactiae and role in biofilm formation - Université de Lorraine Access content directly
Journal Articles Microbial Pathogenesis Year : 2015

Antigen I/II encoded by integrative and conjugative elements of Streptococcus agalactiae and role in biofilm formation

Abstract

Streptococcus agalactiae (i.e. Group B streptococcus, GBS) is a major human and animal pathogen. Genes encoding putative surface proteins and in particular an antigen 1/II have been identified on Integrative and Conjugative Elements (ICEs) found in GBS. Antigens I/II are multimodal adhesins promoting colonization of the oral cavity by streptococci such as Streptococcus gordonii and Streptococcus mu tans. The prevalence and diversity of antigens I/II in GBS were studied by a bioinformatic analysis. It revealed that antigens I/II, which are acquired by horizontal transfer via ICEs, exhibit diversity and are widespread in GBS, in particular in the serotype Ia/ST23 invasive strains. This study aimed at characterizing the impact on GBS biology of proteins encoded by a previously characterized ICE of S. agalactiae (ICE_515-tRNA(Lys)). The production and surface exposition of the antigen I/II encoded by this ICE was examined using RT-PCR and immunoblotting experiments. Surface proteins of ICE_515_tRNA(Lys) were found to contribute to GBS biofilm formation and to fibrinogen binding. Contribution of antigen I/II encoded by SAL 2056 to biofilm formation was also demonstrated. These results highlight the potential for ICEs to spread microbial adhesins between species.
No file

Dates and versions

hal-01475853 , version 1 (24-02-2017)

Identifiers

Cite

Sarah Chuzeville, Shaynoor Dramsi, Jean-Yves Madec, Marisa Haenni, Sophie Payot. Antigen I/II encoded by integrative and conjugative elements of Streptococcus agalactiae and role in biofilm formation. Microbial Pathogenesis, 2015, 88, pp.1 - 9. ⟨10.1016/j.micpath.2015.07.018⟩. ⟨hal-01475853⟩
159 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More