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In vitro impact of physiological shear stress on endothelial cells gene expression profile

Abstract : In the vascular system, the shear applied to the vascular wall activates mechano-sensors located on endothelial cells (ECs) leading to a modification in the gene expression profile. We applied laminar shear stress at 1 Pa on ECs for 6 h and measured by quantitative real time PCR the expression modulation of genes implied in inflammation (ICAM-1 and E-selectin), oxidative stress sensing (HO-1) and vascular tone modulation (eNOS). We showed that all these genes are shear stress inducible. ICAM-1 is more up-regulated than E-selectin suggesting different levels of implication in inflammatory responses and different modes of induction (SSRE, cytokine). Laminar shear stress induces an oxidative stress translated into HO-1 up-regulation, and a possible vasodilatation through the induction of eNOS. Our laminar shear stress system opens a novel and interesting frame in the evaluation of the impact on ECs and blood cells of new pharmacological substances injected in the bloodstream.
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https://hal.univ-lorraine.fr/hal-01493347
Contributor : Caroline Gaucher <>
Submitted on : Thursday, March 23, 2017 - 4:08:47 PM
Last modification on : Friday, May 29, 2020 - 5:10:04 PM

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  • HAL Id : hal-01493347, version 1

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Caroline Gaucher, Carole Devaux, Cédric Boura, Patrick Lacolley, Jean-François Stoltz, et al.. In vitro impact of physiological shear stress on endothelial cells gene expression profile. Clinical Hemorheology and Microcirculation, IOS Press, 2007, Selected Papers from 1st Meeting on “Cardiovascular Biology: Endothelial Cell in Health and Hypertension”, 30 June–1 July 2006, Prague, Czech Republic, 37, pp.99-107. ⟨hal-01493347⟩

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