Ordered mesoporous materials containing Mucor Miehei Lipase as biocatalyst for transesterification reaction

Abstract : Herein, we report the design of a biocatalyst by the immobilization of Mucor Miehei Lipase (Mm-L) onto mesoporous silica materials. Supports with different pore diameters have been considered. Infrared spectroscopy was used to determine the adsorption isotherms in different pH conditions. Then, the biocatalyst was tested for the methanolysis of colza oil. The production of methyl esters was monitored over time by gas chromatography coupled to a mass spectrometer. The results show that to reach maximum performance of the biocatalyst, a certain amount of water is required (5 wt.%). By using ratio lower than the stoichiometry (1:1), the methanol conversion is completed and high transesterification yields could be obtained even in the absence of non polar solvents (i.e. hexane). Herein, the lipase uses the fatty substrate as lipophilic interface required for the opening of the active site of the enzyme.
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Process Biochemistry, Elsevier, 2013, 48 (5-6), pp.831-837. 〈10.1016/j.procbio.2013.04.003〉
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https://hal.univ-lorraine.fr/hal-01494084
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Soumis le : mercredi 22 mars 2017 - 16:40:19
Dernière modification le : jeudi 24 mai 2018 - 10:32:01

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J. Jacoby, Andreea Pasc, C. Carteret, F. Dupire, Marie-José Stebe, et al.. Ordered mesoporous materials containing Mucor Miehei Lipase as biocatalyst for transesterification reaction. Process Biochemistry, Elsevier, 2013, 48 (5-6), pp.831-837. 〈10.1016/j.procbio.2013.04.003〉. 〈hal-01494084〉

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