Determination of iron in calf serum using bathophenanthroline and thermal lens spectrometry

Abstract : Thermal lens spectrometry has been used for the determination of iron in calf serum. The method is based on dissociation of Fe3+ from proteins, reduction of Fe3+ to Fe2+ and formation of a coloured complex between Fe2+ and bathophenanthroline. Contrary to the spectrophotometric method, it is shown that thermal lens spectrometry is less sensitive to scattering caused by the presence of small particles remaining in the test solution after the deproteinization step. The background signal is very small and the response is only slightly dependent on the amount of scattering particles. The method is reliable, sensitive and reproducible. The limit of detection for iron is 4 ppb and the relative standard deviation is around 2%. It is expected that the volume of serum sample necessary for an analysis can be reduced to less than 100 mul.
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Article dans une revue
Analytical Letters, Taylor & Francis, 2000, 33 (15), pp.3153-3163
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https://hal.univ-lorraine.fr/hal-01494606
Contributeur : Sophie Legeai <>
Soumis le : jeudi 23 mars 2017 - 16:49:07
Dernière modification le : samedi 7 avril 2018 - 01:00:49

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  • HAL Id : hal-01494606, version 1

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Sophie Legeai, Joseph Georges. Determination of iron in calf serum using bathophenanthroline and thermal lens spectrometry. Analytical Letters, Taylor & Francis, 2000, 33 (15), pp.3153-3163. 〈hal-01494606〉

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