An l-glucitol oxidizing dehydrogenase from Bradyrhizobium japonicum USDA 110 for production of d-sorbose with enzymatic or electrochemical cofactor regeneration

Abstract : A gene in Bradyrhizobium japonicum USDA 110, annotated as a ribitol dehydrogenase (RDH), had 87 % sequence identity (97 % positives) to the N-terminal 31 amino acids of an l-glucitol dehydrogenase from Stenotrophomonas maltophilia DSMZ 14322. The 729-bp long RDH gene coded for a protein consisting of 242 amino acids with a molecular mass of 26.1 kDa. The heterologously expressed protein not only exhibited the main enantio selective activity with d-glucitol oxidation to d-fructose but also converted l-glucitol to d-sorbose with enzymatic cofactor regeneration and a yield of 90 %. The temperature stability and the apparent K (m) value for l-glucitol oxidation let the enzyme appear as a promising subject for further improvement by enzyme evolution. We propose to rename the enzyme from the annotated RDH gene (locus tag bll6662) from B. japonicum USDA as a d-sorbitol dehydrogenase (EC 1.1.1.14).
Type de document :
Article dans une revue
Applied Microbiology and Biotechnology, Springer Verlag, 2014, 98 (7), pp.3023 - 3032. 〈http://link.springer.com/article/10.1007/s00253-013-5180-7〉. 〈10.1007/s00253-013-5180-7〉
Domaine :
Liste complète des métadonnées

https://hal.univ-lorraine.fr/hal-01497266
Contributeur : Lcpme Ul <>
Soumis le : mardi 28 mars 2017 - 14:46:14
Dernière modification le : jeudi 24 mai 2018 - 10:32:01

Identifiants

Collections

Citation

Sabrina Gauer, Zhijie Wang, Harm Otten, Mathieu Etienne, Morten Jannik Bjerrum, et al.. An l-glucitol oxidizing dehydrogenase from Bradyrhizobium japonicum USDA 110 for production of d-sorbose with enzymatic or electrochemical cofactor regeneration. Applied Microbiology and Biotechnology, Springer Verlag, 2014, 98 (7), pp.3023 - 3032. 〈http://link.springer.com/article/10.1007/s00253-013-5180-7〉. 〈10.1007/s00253-013-5180-7〉. 〈hal-01497266〉

Partager

Métriques

Consultations de la notice

81