Reagentless D-sorbitol biosensor based on D-sorbitol dehydrogenase immobilized in a sol-gel carbon nanotubes-poly(methylene green) composite

Abstract : A reagentless D-sorbitol biosensor based on NAD-dependent D-sorbitol dehydrogenase (DSDH) immobilized in a sol-gel carbon nanotubes-poly(methylene green) composite has been developed. It was prepared by durably immobilizing the NAD(+) cofactor with DSDH in a sol-gel thin film on the surface of carbon nanotubes functionalized with poly(methylene green). This device enables selective determination of D-sorbitol at 0.2 V with a sensitivity of 8.7 mu Ammol(-1)Lcm(-2) and a detection limit of 0.11 mmolL(-1). Moreover, this biosensor has excellent operational stability upon continuous use in hydrodynamic conditions.
Type de document :
Article dans une revue
Analytical and Bioanalytical Chemistry, Springer Verlag, 2013, 405 (11), pp.3899-3906. 〈10.1007/s00216-013-6820-6〉
Domaine :
Liste complète des métadonnées

https://hal.univ-lorraine.fr/hal-01505609
Contributeur : Lcpme Ul <>
Soumis le : mardi 11 avril 2017 - 15:09:01
Dernière modification le : jeudi 24 mai 2018 - 10:32:01

Lien texte intégral

Identifiants

Collections

Citation

Zhijie Wang, Mathieu Etienne, Veronika Urbanova, Gert-Wieland Kohring, Alain Walcarius. Reagentless D-sorbitol biosensor based on D-sorbitol dehydrogenase immobilized in a sol-gel carbon nanotubes-poly(methylene green) composite. Analytical and Bioanalytical Chemistry, Springer Verlag, 2013, 405 (11), pp.3899-3906. 〈10.1007/s00216-013-6820-6〉. 〈hal-01505609〉

Partager

Métriques

Consultations de la notice

72