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Reagentless D-sorbitol biosensor based on D-sorbitol dehydrogenase immobilized in a sol-gel carbon nanotubes-poly(methylene green) composite

Abstract : A reagentless D-sorbitol biosensor based on NAD-dependent D-sorbitol dehydrogenase (DSDH) immobilized in a sol-gel carbon nanotubes-poly(methylene green) composite has been developed. It was prepared by durably immobilizing the NAD(+) cofactor with DSDH in a sol-gel thin film on the surface of carbon nanotubes functionalized with poly(methylene green). This device enables selective determination of D-sorbitol at 0.2 V with a sensitivity of 8.7 mu Ammol(-1)Lcm(-2) and a detection limit of 0.11 mmolL(-1). Moreover, this biosensor has excellent operational stability upon continuous use in hydrodynamic conditions.
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https://hal.univ-lorraine.fr/hal-01505609
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Submitted on : Tuesday, April 11, 2017 - 3:09:01 PM
Last modification on : Friday, February 26, 2021 - 2:42:02 PM

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Zhijie Wang, Mathieu Etienne, Veronika Urbanova, Gert-Wieland Kohring, Alain Walcarius. Reagentless D-sorbitol biosensor based on D-sorbitol dehydrogenase immobilized in a sol-gel carbon nanotubes-poly(methylene green) composite. Analytical and Bioanalytical Chemistry, Springer Verlag, 2013, 405 (11), pp.3899-3906. ⟨10.1007/s00216-013-6820-6⟩. ⟨hal-01505609⟩

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