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Antioxidant and antimicrobial potentials of Retama Sphaerocarpa.

Abstract : The present work studies the antioxidant activities and antimicrobial properties of the aerial part of Retama sphaerocarpa. The secondary metabolites (polyphenols) were extracted using different solvents: chloroform, ethyl acetate and n-butanol. In each fraction, total polyphenols and flavonoids were respectively quantified using Folin-Ciocalteu test and aluminium chloride colorimetric method. The concentration of polyphenols was higher in the chloroform extract (121.4 ± 0.3 mg GAE/g), than in n-butanol extract (111.7 ± 0.1 mg GAE/g) and ethyl acetate extract (73.0 ± 0.4 mgGAE/g). The evaluation of flavonoids content also showed a high value of flavonoid in chloroform extract (20.9 ± 0.2 mg QE /g DM), whereas the ethyl acetate and the n-butanol extracts of Retama sphaerocarpa leaves contained similar quantity of flavonoids with 16.5± 0.8 mg QE /g DM and 15.3± 0.7 mg QE /g DM, respectively. The obtained extracts of R. sphaerocarpa were also evaluated for their antioxidant activities using DPPH radical-scavenging method. The three extracts (i.e. chloroform, ethyl acetate and n-butanol extracts) showed very high free radical scavenging activity: 73 ± 0.8, 75.6 ± 1.9 and 81.5 ± 0.3 (%), respectively. We also investigated the antimicrobial activities of the three extracts using the disc diffusion method. Each of the three extracts were active against Bacillus sp.
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Contributor : Laetitia CANABADY-ROCHELLE Connect in order to contact the contributor
Submitted on : Friday, October 20, 2017 - 12:55:48 PM
Last modification on : Thursday, March 10, 2022 - 3:33:57 PM


  • HAL Id : hal-01620277, version 1


Akila Benaissa, Radia Cherfia, Laetitia Canabady-Rochelle, Dominique Perrin, Patrick Chaimbault, et al.. Antioxidant and antimicrobial potentials of Retama Sphaerocarpa.. Research Journal of Pharmaceutical, Biological and Chemical Sciences, B. M. Vrushabendra Swamy, 2017, 7 (5), pp.214-221. ⟨hal-01620277⟩



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