Microarray-Based Detection and Typing of the Rhizobium Nodulation Gene nodC: Potential of DNA Arrays To Diagnose Biological Functions of Interest - Université de Lorraine Accéder directement au contenu
Article Dans Une Revue Applied and Environmental Microbiology Année : 2005

Microarray-Based Detection and Typing of the Rhizobium Nodulation Gene nodC: Potential of DNA Arrays To Diagnose Biological Functions of Interest

Geoffroy Golfier
  • Fonction : Auteur
Carine Gris-Liebe
  • Fonction : Auteur
Luc Talini
  • Fonction : Auteur
Catherine Boivin-Masson
  • Fonction : Auteur

Résumé

Environmental screening of bacteria for the presence of genes of interest is a challenging problem, due to the high variability of the nucleotide sequence of a given gene between species. Here, we tackle this general issue using a particularly well-suited model system that consists of the nodulation gene nodC, which is shared by phylogenetically distant rhizobia. 41mer and 50mer oligonucleotides featuring the nucleotide diversity of two highly conserved regions of the NodC protein were spotted on glass slides and cross hybridized with the radioactive-labeled target genomic DNA under low-stringency conditions. Statistical analysis of the hybrid-ization patterns allowed the detection of known, as well as new, nodC sequences and classified the rhizobial strains accordingly. The microarray was successfully used to type the nodC gene directly from legume nodules, thus eliminating the need of cultivation of the endosymbiont. This approach could be extended to a panel of diagnostic genes and constitute a powerful tool for studying the distribution of genes of interest in the environment, as well as for bacteria identification.
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Dates et versions

hal-01655599 , version 1 (14-06-2019)

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Citer

Cyril Bontemps, Geoffroy Golfier, Carine Gris-Liebe, Sebastien Carrere, Luc Talini, et al.. Microarray-Based Detection and Typing of the Rhizobium Nodulation Gene nodC: Potential of DNA Arrays To Diagnose Biological Functions of Interest. Applied and Environmental Microbiology, 2005, 71 (12), pp.8042 - 8048. ⟨10.1128/AEM.71.12.8042–8048.2005⟩. ⟨hal-01655599⟩
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