Characterization of Two Families of Spontaneously Amplifiable Units of DNA in Streptomyces ambofaciens - Université de Lorraine Access content directly
Journal Articles Microbiology Year : 1988

Characterization of Two Families of Spontaneously Amplifiable Units of DNA in Streptomyces ambofaciens

Abstract

Four highly amplified DNA sequences (ADS) ranging from 5.8 to 24.8 kb were found in spontaneous mutant strains of Streptomyces ambofaciens DSM 40697. Restriction patterns of total DNA were hybridized with purified ADS6 (24.8 kb) as a probe to detect the amplifiable regions in the wild-type (WT) genome. The results suggested that the amplifiable unit of DNA (AUD) was present as a single copy in the WT genome. Moreover, similarities suggested by the restriction maps of three of the ADS were confirmed by hybridization experiments. The fourth ADS did not hybridize with the three others. Therefore, two families of DNA sequences are potentially amplifiable in the S. ambofaciens genome.
Fichier principal
Vignette du fichier
mic-134-7-2001.pdf (920.39 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-01658578 , version 1 (07-12-2017)

Identifiers

Cite

P. Demuyter, P. Leblond, B. Decaris, J.-M. Simonet. Characterization of Two Families of Spontaneously Amplifiable Units of DNA in Streptomyces ambofaciens. Microbiology, 1988, 134 (7), pp.2001 - 2007. ⟨10.1099/00221287-134-7-2001⟩. ⟨hal-01658578⟩
39 View
101 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More