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Mapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697

Abstract : Streptomyces ambofaciens DSM40697 possess six rRNA gene clusters which can be distinguished by probing BfrI-digested total DNA with the PCR-amplified l6S-23S ribosomal genet internal transcribed spacer. The six rrn loci of S. ambofaciens were cloned as recombinant cosmids and located on the AseI-Dŕal physical map of the linear chromosomal DNA. For five of the six ribosomal gene sets, the transcriptional orientation was determined relative to the physical map and was shown to be divergent away from an oriC-like locus.
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https://hal.univ-lorraine.fr/hal-01658770
Contributor : Pierre LEBLOND Connect in order to contact the contributor
Submitted on : Thursday, December 7, 2017 - 6:04:06 PM
Last modification on : Monday, May 3, 2021 - 5:04:02 PM

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Franca Berger, Gilles Fischer, Adamantini Kyriacou, Bernard Decaris, Pierre Leblond. Mapping of the ribosomal operons on the linear chromosomal DNA of Streptomyces ambofaciens DSM40697. FEMS Microbiology Letters, 1996, pp.167-73. ⟨10.1111/j.1574-6968.1996.tb08476.x⟩. ⟨hal-01658770⟩

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