Physical Map of the Streptomyces lividans 66 Genome and Comparison with That of the Related Strain Streptomyces coelicolor A3(2) - Université de Lorraine Access content directly
Journal Articles Journal of Bacteriology Year : 1993

Physical Map of the Streptomyces lividans 66 Genome and Comparison with That of the Related Strain Streptomyces coelicolor A3(2)

Abstract

A physical map of the chromosome of Streptomyces lividans 66 ZX7 was constructed by ordering the macrorestriction fragments generated from the genomic DNA with the restriction enzymes AseI and DraI. AseI and Dral linking cosmids (i.e., recombinant cosmids including eitherAseI orDral sites) were isolated from a gene bank and used as hybridization probes against Southern transfers of pulsed-field gel electrophoresis (PFGE) restriction patterns. The DraI sites were precisely mapped by PFGE analyses of AseI-DraI double digests and hybridization with the AseI junctions. The 16AseI and 7 Dram fragments were aligned as a single chromosome of about 8,000 kb. The data supported the interpretation that the chromosome is a linear structure. The related strain Streptomyces coelicolor A3(2) M145, recently mapped by H. Kieser, T. Kieser, and D. A. Hopwood (J. Bacteriol. 174:5496-5507, 1992), was compared with S. lividans at the level of the genomic structure by hybridizing the linking cosmids to Southern transfers of PFGE patterns. In spite of little apparent similarity in their restriction patterns, the comparison of the physical maps revealed a common structure with an identical ordering of the cosmid sequences. This conservation of the map order was further confirmed by assigning genetic markers (i.e., cloned genes and DNA elements relevant to the unstable region) to the AseI fragments.
Fichier principal
Vignette du fichier
jbacter00053-0188.pdf (1.96 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-01659214 , version 1 (08-12-2017)

Identifiers

  • HAL Id : hal-01659214 , version 1
  • PUBMED : 8501047

Cite

Pierre Leblond, Matfihias Redenbach, John Cullum. Physical Map of the Streptomyces lividans 66 Genome and Comparison with That of the Related Strain Streptomyces coelicolor A3(2). Journal of Bacteriology, 1993, 175 (11), pp.3422 - 3429. ⟨hal-01659214⟩
50 View
113 Download

Altmetric

Share

Gmail Facebook X LinkedIn More