The Streptomyces lividans 66 chromosome contains a 1 MB deletogenic region flanked by two amplifiable regions - Université de Lorraine Accéder directement au contenu
Article Dans Une Revue Molecular and General Genetics MGG Année : 1993

The Streptomyces lividans 66 chromosome contains a 1 MB deletogenic region flanked by two amplifiable regions

Résumé

Genetic instability in Streptomyces species often involves large deletions sometimes accompanied by DNA amplification. Two such systems in Streptomyces lividans 66 involve the production of mutants sensitive to chloramphenicol and the production of mutants resistant to the galactose analogue 2-deoxygalactose, respectively. Overlapping cosmids were isolated that span the ca. 1 Mb region between the two amplifiable regions. The structure of the region was confirmed by restriction mapping using the rarely cutting enzymes AseI, BfrI and DraI and pulsed-field gel electrophoresis. The region contains a non-clonable gap flanked by inverted repeats; the structure is consistent with the presence of a physical gap, i.e. a linear chromosome.
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Dates et versions

hal-01659218 , version 1 (08-12-2017)

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  • HAL Id : hal-01659218 , version 1
  • PUBMED : 8246878

Citer

R Redenbach, F Flett, P Piendl, I Glocker, R Rauland, et al.. The Streptomyces lividans 66 chromosome contains a 1 MB deletogenic region flanked by two amplifiable regions. Molecular and General Genetics MGG, 1993, 241 (3-4), pp.255-62. ⟨hal-01659218⟩

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