Skip to Main content Skip to Navigation
Journal articles

Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains

Abstract : Streptomyces sp. US 24 and Streptomyces sp. TN 58, two strains producing interesting bioactive molecules, were successfully transformed using E. coli ET12567 (pUZ8002), as a conjugal donor, carrying the integrative plasmid pSET152. For the Streptomyces sp. US 24 strain, two copies of this plasmid were tandemly integrated in the chromosome, whereas for Streptomyces sp. TN 58, the integration was in single copy at the attB site. Plasmid pSET152 was inherited every time for all analysed Streptomyces sp. US 24 and Streptomyces sp. TN 58 exconjugants under nonselective conditions. The growth, morphological differentiation, and active molecules production of all studied pSET152 integrated exconjugants were identical to those of wild type strains. Consequently, conjugal transfer using pSET152 integration system is a suitable means of genes transfer and expression for both studied strains. To validate the above gene transfer system, the glucose isomerase gene (xylA) from Streptomyces sp. SK was expressed in strain Streptomyces sp. TN 58. Obtained results indicated that heterologous glucose isomerase could be expressed and folded effectively. Glucose isomerase activity of the constructed TN 58 recombinant strain is of about eighteenfold higher than that of the Streptomyces sp. SK strain. Such results are certainly of importance due to the potential use of improved strains in biotechnological process for the production of high-fructose syrup from starch.
Document type :
Journal articles
Complete list of metadata

Cited literature [34 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/hal-01659292
Contributor : Pierre Leblond <>
Submitted on : Friday, December 8, 2017 - 11:44:16 AM
Last modification on : Wednesday, April 14, 2021 - 3:14:02 PM

File

464986.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Samiha Sioud, Bertrand Aigle, Ines Karray-Rebai, Slim Smaoui, Samir Bejar, et al.. Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains. Journal of Biomedicine and Biotechnology, Hindawi Publishing Corporation, 2009, 2009 (Article ID 464986), pp.464986. ⟨10.1155/2009/464986⟩. ⟨hal-01659292⟩

Share

Metrics

Record views

217

Files downloads

297