Stimulation of genetic instability in Streptomyces ambofaciens ATCC 23877 by antibiotics that interact with DNA gyrase

Abstract : In wild-type Streptomyces ambofaciens ATCC 23877, pigment-defective (Pig-) mutants arise at a frequency of about 0.5%; this genetic instability is related to genomic rearrangements such as deletions and/or amplifications of DNA sequences. On media containing oxolinic acid and novobiocin, which interact with the A and B subunits of DNA gyrase, respectively, the frequency of variants increased dramatically. The Pig- mutant frequency was increased to almost 100% on a medium containing oxolinic acid at a concentration allowing 55% survival. On solid medium containing either oxolinic acid or novobiocin at subinhibitory concentrations, most colonies exhibited a 'patchwork' phenotype, characterized by the presence of numerous Pig- sectors. Similar phenomena were not observed on media containing the transcriptional inhibitor rifampicin or the translational inhibitor streptomycin. Many of the Pig- mutants exhibited a pleiotropic phenotype and were affected in aerial mycelium formation, colony growth and/or prototrophy. Moreover, the same kinds of rearrangements (deletions and/or amplifications of DNA sequences) were found in both induced and spontaneous Pig- mutants. The results suggest either that DNA gyrase is directly involved in genetic instability or that an SOS-like system is implicated.
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Journal of general microbiology, Society for General Microbiology, (Journal Sales), 1993, 139 (11), pp.2551 - 2558. 〈10.1099/00221287-139-11-2551〉
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Soumis le : vendredi 8 décembre 2017 - 15:35:11
Dernière modification le : lundi 22 janvier 2018 - 14:11:45

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J.-N. Volff, D. Vandewiele, J.-M. Simonet, B. Decaris. Stimulation of genetic instability in Streptomyces ambofaciens ATCC 23877 by antibiotics that interact with DNA gyrase. Journal of general microbiology, Society for General Microbiology, (Journal Sales), 1993, 139 (11), pp.2551 - 2558. 〈10.1099/00221287-139-11-2551〉. 〈hal-01659611〉

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