Skip to Main content Skip to Navigation
New interface
Master thesis

Rôle des terres-rares dans la régulation de l’expression des alcool-déshydrogénases PQQ-dépendantes chez Pseudomonas putida

Abstract : The use of rare-earth element (REEs) as a cofactor for methylotrophic bacteria’s pyrroloquinoline quinone-dependent methanol dehydrogenase (PQQ-dependent MDH) has been a recent discovery, and opens a range of possibilities about the biological role of REEs. The characterization of another PQQ-dependent enzyme, the alcohol-dehydrogenase (PQQADH) REEs-dependent PedH in Pseudomonas putida, shows for the first time the use of the REEs in non-methylotrophic bacteria. The gene of the other PQQ-ADH calcium-dependent from P. putida, pedE, is repressed by REEs. The study reports that REEs are necessary for enzymatic activity and transcriptional regulation of pedE and pedH with low-concentration, in accordance with the environmental concentrations. PedH seems to be involved in the transcriptional regulation of pedE and pedH, and acts as a sensory module. This system uses an inverse regulation of PedE and PedH, which allows a rapid adaptation to its environment, and optimizes its growth with carbon source like alcohol. The regulatory system is also studied using the construction of two mutant libraries.
Complete list of metadata

https://hal.univ-lorraine.fr/hal-01662845
Contributor : Memoires UL Connect in order to contact the contributor
Submitted on : Wednesday, December 13, 2017 - 2:52:41 PM
Last modification on : Saturday, April 30, 2022 - 4:03:26 PM

Intranet access

Identifiers

  • HAL Id : hal-01662845, version 1

Collections

Citation

Audrey Martin-Mériadec. Rôle des terres-rares dans la régulation de l’expression des alcool-déshydrogénases PQQ-dépendantes chez Pseudomonas putida. Microbiologie et Parasitologie. 2017. ⟨hal-01662845⟩

Share

Metrics

Record views

75