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Master thesis

Rôle des terres-rares dans la régulation de l’expression des alcool-déshydrogénases PQQ-dépendantes chez Pseudomonas putida

Abstract : The use of rare-earth element (REEs) as a cofactor for methylotrophic bacteria’s pyrroloquinoline quinone-dependent methanol dehydrogenase (PQQ-dependent MDH) has been a recent discovery, and opens a range of possibilities about the biological role of REEs. The characterization of another PQQ-dependent enzyme, the alcohol-dehydrogenase (PQQADH) REEs-dependent PedH in Pseudomonas putida, shows for the first time the use of the REEs in non-methylotrophic bacteria. The gene of the other PQQ-ADH calcium-dependent from P. putida, pedE, is repressed by REEs. The study reports that REEs are necessary for enzymatic activity and transcriptional regulation of pedE and pedH with low-concentration, in accordance with the environmental concentrations. PedH seems to be involved in the transcriptional regulation of pedE and pedH, and acts as a sensory module. This system uses an inverse regulation of PedE and PedH, which allows a rapid adaptation to its environment, and optimizes its growth with carbon source like alcohol. The regulatory system is also studied using the construction of two mutant libraries.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/ulprive/BUS_M_2017_MARTIN_MERIADEC_AUDREY.pdf
Complete list of metadatas

https://hal.univ-lorraine.fr/hal-01662845
Contributor : Memoires Ul <>
Submitted on : Wednesday, December 13, 2017 - 2:52:41 PM
Last modification on : Monday, October 19, 2020 - 2:41:35 PM

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  • HAL Id : hal-01662845, version 1

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Citation

Audrey Martin-Mériadec. Rôle des terres-rares dans la régulation de l’expression des alcool-déshydrogénases PQQ-dépendantes chez Pseudomonas putida. Microbiologie et Parasitologie. 2017. ⟨hal-01662845⟩

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