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A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP

Abstract : The box C/D snoRNAs function in directing 2'-O-methylation and/or as chaperones in the processing of ribosomal RNA. We show here that Snu13p (15.5 kD in human), a component of the U4/U6.U5 tri-snRNP, is also associated with the box C/D snoRNAs. Indeed, genetic depletion of Snu13p in yeast leads to a major defect in RNA metabolism. The box C/D motif can be folded into a stem-internal loop-stem structure, almost identical to the 15.5 kD binding site in the U4 snRNA. Consistent with this, the box C/D motif binds Snu13p/ 15.5 kD in vitro. The similarities in structure and function observed between the U4 snRNP (chaperone for U6) and the box C/D snoRNPs raises the interesting possibility that these particles may have evolved from a common ancestral RNP.
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https://hal.univ-lorraine.fr/hal-01704615
Contributor : Bruno Charpentier <>
Submitted on : Thursday, February 8, 2018 - 3:44:02 PM
Last modification on : Monday, December 14, 2020 - 3:46:14 PM

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Nicholas J Watkins, Véronique Senty-Ségault, Bruno Charpentier, Stéphanie Nottrott, Patricia Fabrizio, et al.. A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP. Cell, Elsevier, 2000, 103 (3), pp.457-66. ⟨10.1016/S0092-8674(00)00137-9⟩. ⟨hal-01704615⟩

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