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Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro

Abstract : Objective: The efficacy of glucosamine sulfate (GS) in the symptomatic treatment of patients with osteoarthritis (OA) is suggested to be mediated by still unknown effects on the altered OA cartilage. Design: Using human OA chondrocytes in culture, the effects of GS on protein synthesis, caseinase, collagenase, phospholipase A2 (PLA2) and protein kinase C (PKC) activities as well as production of nitric oxide and cyclic AMP were studied in both cells and culture medium. Results: GS significantly reduced PLA2 activity, and more modestly collagenase activity, in the OA chondrocytes in a dose-dependent manner. By contrast, PLA2 and collagenase activity of the culture medium was not modified. No effects on caseinase activity was seen. GS significantly and dose-dependently increased protein synthesis. GS did not modify nitric oxide and cAMP production but significantly increased PKC production. Conclusion: GS modified cultured OA chondrocyte metabolism by acting on PKC, cellular PLA2, protein synthesis and possibly collagenase activation. Extrapolation of the effect to the in-vivo situation remains hypothetical but they might represent some possible mechanisms of action of the drug in human.
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https://hal.univ-lorraine.fr/hal-01705629
Contributor : Pascal Reboul <>
Submitted on : Friday, February 9, 2018 - 5:22:53 PM
Last modification on : Friday, March 12, 2021 - 2:32:01 PM

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M. Piperno, P. Reboul, M Hellio Le Graverand, M Peschard, M Annefeld, et al.. Glucosamine sulfate modulates dysregulated activities of human osteoarthritic chondrocytes in vitro. Osteoarthritis and Cartilage, Elsevier, 2000, 8 (3), pp.207 - 212. ⟨10.1053/joca.1999.0291⟩. ⟨hal-01705629⟩

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