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Study of retinoic acid effect upon retinoic acid receptors beta (RAR-beta) in C6 cultured glioma cells.

Abstract : Using monoclonal antibodies against the RAR-alpha and RAR-beta retinoic receptors, we demonstrated that these receptors were present together in C6 glioma cells as two isoforms of 50 and 55 kDa. For RAR-beta, the 50 kDa isoform predominated (60 to 80% of the total of the two isoforms). After a treatment for 48 h with retinoic acid 10 microM, the 55 kDa form was enhanced, while no effect was observed either on RAR-alpha isoforms from C6 cells and on both RAR-alpha and RAR-beta forms from neuroblastoma SKN SH SY5Y used as a control. Using purified neuronal and glial rat brain nuclei, we showed that the 55 kDa isoform from RAR-beta predominated in glial cells. These results suggest that retinoic acid treatment of C6 cells led to a partial differentiation, the enhancement of the heavy form of RAR-beta being a marker of this phenomenon.
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https://hal.univ-lorraine.fr/hal-01705678
Contributor : Pascal Reboul <>
Submitted on : Friday, February 9, 2018 - 4:25:39 PM
Last modification on : Monday, June 1, 2020 - 5:02:05 AM

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  • HAL Id : hal-01705678, version 1
  • PUBMED : 7581005

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P. Reboul, P George, P. Louisot, P. Broquet. Study of retinoic acid effect upon retinoic acid receptors beta (RAR-beta) in C6 cultured glioma cells.. Biochemistry and molecular biology international, 1995, pp.1097-105. ⟨hal-01705678⟩

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