Assay of circulating hyaluronic acid in the rat: study of diurnal variation and effect of anesthesia. - Université de Lorraine Accéder directement au contenu
Article Dans Une Revue Life Sciences Année : 1991

Assay of circulating hyaluronic acid in the rat: study of diurnal variation and effect of anesthesia.

Résumé

Serum hyaluronic acid (HA) may provide a good marker for the severity of joint disease in the rat since a positive correlation was observed in experimental models of arthritis. However, little is known about its physiological variation in rats. In the present work, we do not find any circadian rhythm of HA in healthy Sprague-Dawley rats in contrast to that observed in humans, whose serum levels vary during daytime. Furthermore, the influence of blood sampling conditions on HA concentrations was evaluated in conscious animals and by using different anesthetics. The greater reproducibility for the assay of HA is observed with the intracardiac puncture under ether inhalation. Blood sample collection in the absence of anesthesia leads to a significant increase in serum levels of HA, which could be attributed partly to enhanced joint movements generated by psychological stress.
Fichier non déposé

Dates et versions

hal-01707327 , version 1 (12-02-2018)

Identifiants

  • HAL Id : hal-01707327 , version 1
  • PUBMED : 1865756

Citer

P. Gegout, D Drelon, J Jouzeau, P Payan, D Chevrier, et al.. Assay of circulating hyaluronic acid in the rat: study of diurnal variation and effect of anesthesia.. Life Sciences, 1991, pp.PL31-5. ⟨hal-01707327⟩

Collections

CNRS UNIV-LORRAINE
29 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More