Assay of synovial fluid hyaluronic acid using high-performance liquid chromatography of hyaluronidase digests. - Université de Lorraine Accéder directement au contenu
Article Dans Une Revue Journal of Chromatography B Biomedical Sciences and Applications Année : 1991

Assay of synovial fluid hyaluronic acid using high-performance liquid chromatography of hyaluronidase digests.

Résumé

A high-performance liquid chromatographic method for the determination of hyaluronic acid levels in synovial fluids has been developed. The hyaluronidase sample digests, containing an internal standard (benzoic acid), were separated on a reversed-phase octadecylsilyl column eluted with 0.01 M tetrabutylammonium phosphate-acetonitrile (83:17, v/v) at pH 7.35. The determination was made on 1:10 diluted samples, by using a calibration curve from 50 to 500 micrograms/ml of human umbilical cord hyaluronic acid. For validation, the synovial fluids were simultaneously analysed by this method and a radiometric method: a high correlation was found between the two (correlation coefficient 0.94). The proposed method can be used to determine specifically the high hyaluronic acid levels of synovial fluids without interferences from other glycosaminoglycans or non-steroidal anti-inflammatory drug treatment.
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Dates et versions

hal-01707403 , version 1 (12-02-2018)

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  • HAL Id : hal-01707403 , version 1
  • PUBMED : 1885725

Citer

P Payan, J-y Jouzeau, F. Lapicque, N Muller, P. Gegout, et al.. Assay of synovial fluid hyaluronic acid using high-performance liquid chromatography of hyaluronidase digests.. Journal of Chromatography B Biomedical Sciences and Applications, 1991, pp.9-18. ⟨hal-01707403⟩

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