Métabolisme de l'ARN chez les archées : caractérisation de la protéine issue du gène PAB1868

Abstract : Today, only a few ribonucleases involved in degradation and RNA processing have been observed in archaea. All these proteins have exclusively an exonucleolytic activity. Here we show that a Metallo-beta-lactamase-type beta-CASP protein: a homologue of the the human's CPSF-73 protein (Cleavage and polyadenylation Specific Factor) and expressed in Pyrococcus abyssi presents endonucleolytic activity. We called this protein Pab-CPSF but it was originally annotated Pab-1868. The Endonucleolytic activity of this protein is dependent on a specific CA pattern just as the one required for the activity of human's CPSF-73. However this only sequence specificity does not seem sufficient to observe any activity. We also observed differences in activity depending on the 5 'end of RNA, but this remains to be determined. This protein can also present an exonucleolytic activity which remains much less processive than the RNA.J's homologue's activity. In addition we have studied deletion mutants and then we are able to show that the KH domain (binding RNA fold) is not necessarily recquired for the protein's activity. In the same time a bioinformatics' study has been carried out and it shows that archaea possess three homologues of CPSF-73 protein, but only Pab-CPSF can be found among all archaea.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/prive/SCDSCI_M_2011_ANGLES_FREDERIC.pdf
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https://hal.univ-lorraine.fr/hal-01798935
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Submitted on : Thursday, May 24, 2018 - 10:19:31 AM
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Frédéric Angles. Métabolisme de l'ARN chez les archées : caractérisation de la protéine issue du gène PAB1868. Sciences de l'environnement. 2011. ⟨hal-01798935⟩

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