Occurrence, Survival, and Persistence of Human Adenoviruses and F-Specific RNA Phages in Raw Groundwater - Archive ouverte HAL Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2010

Occurrence, Survival, and Persistence of Human Adenoviruses and F-Specific RNA Phages in Raw Groundwater

(1) , (1) , (2) , (3) , (1)
1
2
3

Abstract

Detection of specific genetic markers can rapidly identify the presence of enteric viruses in groundwater. However, comparison of stability characteristics between genetic and infectivity markers is necessary to better interpret molecular data. Human adenovirus serotype 2 (HAdV2), in conjunction with MS2 phages or GA phages, was spiked into raw groundwater microcosms. Viral stability was periodically assessed by both infectivity and real-time PCR methods. The results of this yearlong study suggest that adenoviruses have the most stable persistence profile and an ability to survive for a long time in groundwater. According to a linear regression model, infectivity reductions of HAdV2 ranged from 0.0076 log(10)/day (4°C) to 0.0279 log(10)/day (20°C) and were significantly lower than those observed for phages. No adenoviral genome degradation was observed at 4°C, and the reduction was estimated at 0.0036 log(10)/day at 20°C. Occurrence study showed that DNA of human adenoviruses could be observed in groundwater from a confined aquifer (7 of the 60 samples were positive by real-time PCR), while no fecal indicators were detected. In agreement with the persistence of genetic markers, the presence of adenoviral DNA in groundwater may be misleading in term of health risk, especially in the absence of information on the infective status.
Fichier principal
Vignette du fichier
Ogorzaly et al., 2010.pdf (270.38 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-01802257 , version 1 (29-05-2018)

Identifiers

Cite

Leslie Ogorzaly, Isabelle Bertrand, M. Paris, A. Maul, Christophe Gantzer. Occurrence, Survival, and Persistence of Human Adenoviruses and F-Specific RNA Phages in Raw Groundwater. Applied and Environmental Microbiology, 2010, 76 (24), pp.8019 - 8025. ⟨10.1128/AEM.00917-10⟩. ⟨hal-01802257⟩
69 View
116 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More