Detection and genotyping of Giardia duodenalis in wastewater: Relation between assemblages and faecal contamination origin

Abstract : Among the seven assemblages identified in Giardia duodenalis species, only assemblages A and B infect humans and numerous other mammals as well. On the other hand, assemblage E is considered to be host restricted to livestock. The aim of the present study was to compare the presence of G. duodenalis assemblages A, B and E in wastewater samples from two municipal treatment plants (n=24) and one slaughterhouse (n=12). Thus, PCR assays targeting the tpi gene were developed to detect specifically these three G. duodenalis assemblages. Assemblages A and B were detected in urban wastewater with a predominance of assemblage A, especially for one treatment plant. Concerning slaughterhouse wastewater, assemblage A was found in 58% of the samples, whereas assemblage B was not detected. Assemblage E was not detected in urban wastewater, but was found in 92% of the samples from slaughterhouse. Thus, combination of assemblages A and B seemed to indicate a human contamination origin, while combination of assemblages A and E appeared to correspond to a livestock contamination origin.
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https://hal.univ-lorraine.fr/hal-01802347
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Submitted on : Tuesday, May 29, 2018 - 11:19:33 AM
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Isabelle Bertrand, Janine Schwartzbrod. Detection and genotyping of Giardia duodenalis in wastewater: Relation between assemblages and faecal contamination origin. Water Research, IWA Publishing, 2007, 41 (16), pp.3675 - 3682. ⟨10.1016/j.watres.2007.02.043⟩. ⟨hal-01802347⟩

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