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Master thesis

Influence of carbon starvation and of TOR inhibition on Arabidopsis thaliana cell cultures

Abstract : Carbohydrates are major molecules in plant development. They have multiple roles: they have a vital energy producing role, a structural importance by contributing to the cell wall synthesis and a signaling function. This signaling role is crucial and helps plants to adapt their metabolism to varying conditions in their environment. To relay this information some sensors are required. One of them is a kinase called TOR (Target Of Rapamycin) which is known to be upregulated by the presence of sugars. TOR activity stimulates growth, transcription and nutrient uptake. This kinase is activated in favorable condition and switched off by any kind of stress. Nevertheless TOR is not the only kinase which take part of carbon signaling and some responses to a carbon starvation are totally independent from it. This works propose to determine the metabolic and transciptomic responses to a carbon starvation and TOR inhibition and beyond them which are connected together. Arabidopsis thaliana cell cultures were used as model for their homogeneity and their ability to grow fast. A study of a new protocol to transform those cells is included. This study would lead to a better understanding of this TOR-sugars interaction. It could be use in the future to increase metabolite content in cell cultures like microalgae by adjusting the activity of TOR which could help for energy production.
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Master thesis
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http://docnum.univ-lorraine.fr/public/BUS_M_2016_GAUTHIER_KEVIN.pdf
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https://hal.univ-lorraine.fr/hal-01824662
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Kevin Gauthier. Influence of carbon starvation and of TOR inhibition on Arabidopsis thaliana cell cultures. Sciences de l'environnement. 2016. ⟨hal-01824662⟩

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