Influence des conditions de traitement d’échantillons de bioaérosols sur les résultats de dosage des (1,3)-bêta-D-glucanes

Abstract : (1,3)-bêta-D-glucanes are glucose polymers connected with (1,3) linkage. They can adopt different conformations: linear, circular or branched. They are the major component of fungi cell wall but also found in some plants or bacteria. Glucan airborne exposure can be associated with upper airway inflammation or atopy. This kind of biopolymers can be assayed using the LAL method. Influence of six treatments on the assay has been tested (untreated, cell grinding, autoclaving, sonication, alkalin treatment and Tween 80 treatment). We used two cell suspension models; S. cerevisiae and P. brevicompactum; and three pure glucans models; glucans from barley, laminarin and schizophyllan). The most efficient treatment was cell grinding in addition to Tween 80 treatment. Additional tests will be performed to optimize these treatments. For good practice, each material has to be decontaminated using sonication in ethanol for two hours.
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Master thesis
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Jodelle Degois. Influence des conditions de traitement d’échantillons de bioaérosols sur les résultats de dosage des (1,3)-bêta-D-glucanes. Microbiologie et Parasitologie. 2014. ⟨hal-01838121⟩

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