Skip to Main content Skip to Navigation
New interface
Journal articles

Potential relationship between glutathione metabolism and flocculation in the yeast Kluyveromyces lactis

Abstract : Reduced glutathione (GSH) is involved in biochemical and physiological processes in cells. Flocculation is an important mechanism in The present study concerned the potential relationship between GSH metabolism and flocculation. Two yeast strains, a flocculent (Kluyveromyces lactis 5c) and a nonflocculent (Kluyveromyces lactis 5a) strain, were used. The level of intracellular GSH measured during the growth period was significantly higher in the nonflocculent than in the flocculent strain; in contrast, the flocculent strain exhibited brighter staining of vacuoles than the nonflocculent strain when observed using epifluorescence microscopy. Compounds acting either on flocculation (EDTA, galactose) or on GSH metabolism (buthionine sulfoximine, and N-acetylcysteine) were tested on the flocculent strain during the growth period. Both EDTA and galactose fully inhibited flocculation and induced GSH overproduction of 58% and 153%, respectively. Buthionine sulfoximine decreased GSH level by 76% but had no effect on flocculation; N-acetylcysteine increased the GSH level and flocculation by 106% and 41%, respectively. Combination of EDTA and N-acetylcysteine produced similar effects than with each of them. Combination of galactose and N-acetylcysteine increased the GSH level but decreased flocculation. These results demonstrated that GSH homeostasis is linked to the flocculation mechanism. A hypothesis related to stress is given.
Document type :
Journal articles
Complete list of metadata
Contributor : LCPME UL Connect in order to contact the contributor
Submitted on : Wednesday, July 25, 2018 - 9:25:36 AM
Last modification on : Thursday, December 9, 2021 - 2:54:07 PM

Intranet access




Joël Coulon, Lydia Matoub, M. Dossot, Stéphanie Marchand, Grzegorz Bartosz, et al.. Potential relationship between glutathione metabolism and flocculation in the yeast Kluyveromyces lactis. FEMS Yeast Research, 2007, 7 (1), pp.93 - 101. ⟨10.1111/j.1567-1364.2006.00146.x⟩. ⟨hal-01848654⟩



Record views