Skip to Main content Skip to Navigation

Monitoring the Dissemination of the Broad-Host-Range Plasmid pB10 in Sediment Microcosms by Quantitative PCR

Abstract : Studying the transfer of specific mobile genetic elements in complex environmental matrices remains difficult because suitable molecular tools are not yet available to back up classical culture-dependent approaches. In this report, we show that quantitative PCR could be used to monitor the dissemination of the broad-host-range plasmid pB10 in sediment microcosms. This approach lies in the differential measurement of the host and plasmid DNAs used to inoculate the microcosms, using a particular design of quantitative PCR primers/probes where we took advantage of the mosaic aspect of the bacterial genomes to achieve a highly specific quantitative PCR detection system.
Document type :
Journal articles
Complete list of metadatas

https://hal.univ-lorraine.fr/hal-01849624
Contributor : Lcpme Ul <>
Submitted on : Thursday, July 26, 2018 - 1:36:39 PM
Last modification on : Friday, July 27, 2018 - 1:16:12 AM

Links full text

Identifiers

Collections

Citation

S. Bonot, C. Merlin. Monitoring the Dissemination of the Broad-Host-Range Plasmid pB10 in Sediment Microcosms by Quantitative PCR. Applied and Environmental Microbiology, American Society for Microbiology, 2009, 76 (1), pp.378 - 382. ⟨10.1128/AEM.01125-09⟩. ⟨hal-01849624⟩

Share

Metrics

Record views

43