HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

The impact of chlorine and heat on the infectivity and physicochemical properties of bacteriophage MS2

Abstract : Enteric viruses and bacteriophages are exposed to various inactivating factors outside their host, and among them chlorine and heat are the most commonly used sanitizer in water industry and treatment in the food industry, respectively. Using MS2 phages as models for enteric viruses, we investigated the impact of free chlorine and heat on their physicochemical properties. Free chlorine was first evaluated alone. No increase in either capsid permeability or hydrophobicity was observed. The negative surface charge slightly increased suggesting molecular changes in the capsid. However, a weakening of the capsid by chlorine was suggested by differential scanning fluorimetry. This phenomenon was confirmed when chlorination was followed by a heat treatment. Indeed, an increase in the inactivation of MS2 phages and the permeability of their capsids to RNases was observed. More interestingly, an increase in the expression of hydrophobic domains at the phage surface was observed, but only for phages remaining infectious. The chlorine-caused weakening of the capsid suggested that, for an optimal use, the oxidant should be followed by heat. The increased permeability to RNases and the expression of hydrophobic domains may contribute to the development or improvement of molecular methods specific for infectious enteric viruses.
Complete list of metadata

Cited literature [52 references]  Display  Hide  Download

Contributor : Isabelle Bertrand Connect in order to contact the contributor
Submitted on : Sunday, May 5, 2019 - 9:06:40 PM
Last modification on : Tuesday, January 4, 2022 - 4:02:17 AM


Brié et al., 2018 FEMSEC.pdf
Files produced by the author(s)




Adrien Brié, C. Gantzer, Nicolas Boudaud, Isabelle Bertrand. The impact of chlorine and heat on the infectivity and physicochemical properties of bacteriophage MS2. FEMS Microbiology Ecology, Wiley-Blackwell, 2018, 94 (8), pp.fiy106. ⟨10.1093/femsec/fiy106⟩. ⟨hal-01867893⟩



Record views


Files downloads