Enterovirus genome detection in wastewater: multi centric evaluation of a commercial kit

Abstract : A multi-centric study was carried out in three laboratories, to evaluate the efficiency of a standardized kit for the detection of enterovirus genome in wastewater. Twenty one samples of 20 liters of wastewater were analyzed before and after concentration through glass wool. Each sample was analyzed with the Amplicor® kit as well as with techniques developed independently in each laboratory. The results show that the Amplicor® kit is well suited to the detection of enterovirus genome in treated wastewater. The results may be compared to those obtained with semi-nested RT-PCR techniques used in each laboratory. However, the Amplicor® kit technique is more simple and has the advantage of providing a standardized technique useful for comparative studies. During this work it was observed that the sensitivity of the detection of infectious viruses and virus genome was improved when concentrated samples were used for analysis.
Complete list of metadatas

https://hal.univ-lorraine.fr/hal-01890647
Contributor : Christophe Gantzer <>
Submitted on : Monday, October 8, 2018 - 6:28:11 PM
Last modification on : Tuesday, November 13, 2018 - 4:06:13 PM

Links full text

Identifiers

Citation

C. Gantzer, D. Menard, B. Lina, A. Maul, S. Le Guyader, et al.. Enterovirus genome detection in wastewater: multi centric evaluation of a commercial kit. Zentralblatt für Hygiene und Umweltmedizin, 1999, 202 (1), pp.29 - 39. ⟨10.1016/S0934-8859(99)80050-9⟩. ⟨hal-01890647⟩

Share

Metrics

Record views

54