Amperometric biosensor for the D-sorbitol determination based on glassy carbon electrode modified with biocomposite film - Université de Lorraine Access content directly
Journal Articles Metody i Ob'ekty Khimicheskogo Analiza = Methods and Objects of Chemical Analysis Year : 2012

Amperometric biosensor for the D-sorbitol determination based on glassy carbon electrode modified with biocomposite film

Abstract

The method of electrophoretic deposition is used here for the obtaining a carbon nanotubes layer on the surface of glassy carbon electrode. The electrode modified with carbon nanotubes demonstrated significant shift of the oxidation potential of co-factor NADH (ΔЕ = 0.25 V) and enhanced stability of the electrochemical response compare to non-modified electrode. The D-sorbitol dehydrogenase enzyme was immobilized into the silica film on the surface of carbon nanotubes-modified electrode. The developed biocomposite electrode was applied for the D-sorbitol determination in foodstuffs and cosmetics with the limit of detection 0.1 mM. The prospects of application of developed electrode as a sensitive element of amperometric biosensor are discussed.
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Dates and versions

hal-01941823 , version 1 (02-12-2018)

Identifiers

  • HAL Id : hal-01941823 , version 1

Cite

Ievgen Mazurenko, O Shevchenko, Oksana Tananaiko, Mathieu Etienne, Alain Walcarius. Amperometric biosensor for the D-sorbitol determination based on glassy carbon electrode modified with biocomposite film. Metody i Ob'ekty Khimicheskogo Analiza = Methods and Objects of Chemical Analysis, 2012, 7 (4), pp.176-183. ⟨hal-01941823⟩
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