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Amperometric biosensor for the D-sorbitol determination based on glassy carbon electrode modified with biocomposite film

Abstract : The method of electrophoretic deposition is used here for the obtaining a carbon nanotubes layer on the surface of glassy carbon electrode. The electrode modified with carbon nanotubes demonstrated significant shift of the oxidation potential of co-factor NADH (ΔЕ = 0.25 V) and enhanced stability of the electrochemical response compare to non-modified electrode. The D-sorbitol dehydrogenase enzyme was immobilized into the silica film on the surface of carbon nanotubes-modified electrode. The developed biocomposite electrode was applied for the D-sorbitol determination in foodstuffs and cosmetics with the limit of detection 0.1 mM. The prospects of application of developed electrode as a sensitive element of amperometric biosensor are discussed.
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https://hal.univ-lorraine.fr/hal-01941823
Contributor : Alain Walcarius <>
Submitted on : Sunday, December 2, 2018 - 3:35:49 PM
Last modification on : Tuesday, February 4, 2020 - 10:38:08 AM

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  • HAL Id : hal-01941823, version 1

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Ievgen Mazurenko, O Shevchenko, Oksana Tananaiko, Mathieu Etienne, Alain Walcarius. Amperometric biosensor for the D-sorbitol determination based on glassy carbon electrode modified with biocomposite film. Metody i Ob'ekty Khimicheskogo Analiza = Methods and Objects of Chemical Analysis, Analytical Chemistry Scientific Council of Ukraine, 2012, 7 (4), pp.176-183. ⟨hal-01941823⟩

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