The method of electrophoretic deposition is used here for the obtaining a carbon nanotubes layer on the surface of glassy carbon electrode. The electrode modified with carbon nanotubes demonstrated significant shift of the oxidation potential of co-factor NADH (ΔЕ = 0.25 V) and enhanced stability of the electrochemical response compare to non-modified electrode. The D-sorbitol dehydrogenase enzyme was immobilized into the silica film on the surface of carbon nanotubes-modified electrode. The developed biocomposite electrode was applied for the D-sorbitol determination in foodstuffs and cosmetics with the limit of detection 0.1 mM. The prospects of application of developed electrode as a sensitive element of amperometric biosensor are discussed.