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Electrochemically assisted deposition of sol–gel bio-composite with co-immobilized dehydrogenase and diaphorase

Abstract : We report here that the electrochemically assisted deposition (EAD) of silica thin films can be a good strategy to co-encapsulate d-sorbitol dehydrogenase (DSDH) and diaphorase in an active form. This is achieved via the electrolysis of a hydrolyzed sol containing the biomolecules to initiate the poly-condensation of silica precursors upon electrochemically induced pH increase at the electrode/solution interface. DSDH was found to be very sensitive to the silica gel environment and the addition of a positively-charged polyelectrolyte was necessary to ensure effective operational behavior of the biomolecules. The composition of the sol and the conditions for electrolysis have been optimized with respect to the intensity of the electrochemical response to d-sorbitol oxidation. The Km of DSDH in the electrodeposited film was in the range of 3 mM, slightly better than the value determined biochemically in solution (6.5 mM). The co-immobilization of DSDH and diaphorase in this way led on the one hand to the possible reduction of NAD+ to NADH (simultaneously to d-sorbitol oxidation) and on the other hand to the safe re-oxidation of the co-factor using a mediator (ferrocenedimethanol) as electron relay. The bioelectrocatalytic response looks promising for electro-enzymatic applications. To support this idea, the EAD of sol–gel bio-composite has been extended to macroporous electrodes displaying a much bigger electroactive surface area.
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Submitted on : Sunday, December 2, 2018 - 5:21:17 PM
Last modification on : Thursday, December 9, 2021 - 2:54:07 PM



Zhijie Wang, Mathieu Etienne, Gert-Wieland Kohring, Yémima Bon-Saint-Côme, Alexander Kuhn, et al.. Electrochemically assisted deposition of sol–gel bio-composite with co-immobilized dehydrogenase and diaphorase. Electrochimica Acta, 2011, 56 (25), pp.9032-9040. ⟨10.1016/j.electacta.2011.05.130⟩. ⟨hal-01941867⟩



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