Preparing Catalytic Surfaces for Sensing Applications by Immobilizing Enzymes via Hydrophobin Layers - Université de Lorraine Accéder directement au contenu
Article Dans Une Revue Analytical Chemistry Année : 2005

Preparing Catalytic Surfaces for Sensing Applications by Immobilizing Enzymes via Hydrophobin Layers

Yohann Corvis
Rick Rink
  • Fonction : Auteur
Nadir Mrabet
  • Fonction : Auteur
Ewa Rogalska

Résumé

Simple and reliable immobilization techniques that preserve the activity of enzymes are of interest in many technologies based on catalysis. Here, two redox enzymes, glucose oxidase from Aspergillus niger and horseradish peroxidase, were immobilized by physisorption on glassy carbon electrodes coated with Schizophyllum commune hydrophobin. Hydrophobins are small, interfacially active proteins that have the remarkable property of adhering to almost any surface. We showed recently that these proteins can be used to immobilize small, electroactive molecules. The results obtained in this work show a way to easily manufacture stable, enzyme-based catalytic surfaces for applications in biosensing.

Dates et versions

hal-01941970 , version 1 (02-12-2018)

Identifiants

Citer

Yohann Corvis, Alain Walcarius, Rick Rink, Nadir Mrabet, Ewa Rogalska. Preparing Catalytic Surfaces for Sensing Applications by Immobilizing Enzymes via Hydrophobin Layers. Analytical Chemistry, 2005, 77 (6), pp.1622-1630. ⟨10.1021/ac048897w⟩. ⟨hal-01941970⟩
22 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More