Détection de gènes de résistance aux antibiotiques dans les bactéries isolées des produits de la mer

Abstract : Nowadays, bacterial antimicrobial resistance constitutes a challenge in public health. In the European Union surveillance systems monitor antimicrobial resistance in terrestrial food animals: bovine, pigs and poultry. No monitoring system currently exists for seafood even if the consumption of seafood is important (34.5 kg/inh/year consumed in France and 20 kg/inh/year worldwide). In this study we developed a high-throughput qPCR chip to detect antimicrobial resistance genes with a critical importance in human health. We validated qPCR tests targeting 40 antimicrobial resistance genes for Gram-negative bacteria. This method allowed us to detect four antimicrobial resistance genes and one plasmid-associated gene in a collection of 159 Gram-negative bacterial strains isolated from seafood products. This tool will be useful to follow the introduction and dissemination of antimicrobial resistance genes in the food chain.
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Master thesis
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Emilie Dauvergne. Détection de gènes de résistance aux antibiotiques dans les bactéries isolées des produits de la mer. Microbiologie et Parasitologie. 2018. ⟨hal-02010604⟩

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