Génogroupage des bactériophages ARN F-spécifiques pour discriminer l'origine humaine ou animale d'une pollution fécale dans les eaux

Abstract : F-specific RNA phages are used to estimate viral pollution due to their persistence in environmental waters. They may also be interesting for tracking the origin of fecal pollution by the distribution of 4 genogroups in water. Members of groups II and III are highly associated with human fecal contamination, while group I and IV have higher incidence in wastes associated with animals and livestock, but this distribution is not absolute. First, we have studied the distribution of phages in wastewater slaughterhouse (bovine pollution) and in wastewater treatment plant (human pollution). Phages of genogroup III are largely identified in both matrices, limiting their discriminating power. Then, in order to increase the specificity of these indicators, we have sequenced phages of different origins. It has appeared that within a genogroup, populations of genetically different viruses are hostspecific. Finally, a persistence study confirmed the longest stability of the genome of phages in wastewater relative to the infectivity; the more persistent genomes are those of the genogroups I and II phages.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/prive/BUS_M_2014_HARTARD_CEDRIC.pdf
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https://hal.univ-lorraine.fr/hal-02097513
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Submitted on : Friday, April 12, 2019 - 9:46:39 AM
Last modification on : Saturday, April 13, 2019 - 1:25:04 AM

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Cédric Hartard. Génogroupage des bactériophages ARN F-spécifiques pour discriminer l'origine humaine ou animale d'une pollution fécale dans les eaux. Microbiologie et Parasitologie. 2014. ⟨hal-02097513⟩

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