occurring in such plants as Leucojum aestivum, are currently of great medical importance. Endophytes are microorganisms living in plants without causing any damage for the plant. Recently, a trend in the biotechnology is the possibility to use the ability of certain endophytic bacteria to produce medicinal substances as well as their stimulating effect on the biosynthesis of secondary metabolites under in vitro conditions. The objective of this project is to determine the possibilities of using endophytic bacteria colonizing Leucojum aestivum for the production of Amaryllidaceae alkaloids.