Production de la Kinamycine chez Streptomyces ambofaciens : caractérisation de la molécule signal régulatrice et de la voie de la biosynthèse.

Abstract : In Streptomyces ambofaciens ATCC 23877 the alp biosynthetic cluster is responsible for the production of kinamycin. It is controlled by a complex regulatory network that also includes a quorum sensing (QS) mechanism. The synthesis of the signaling molecule involved in the QS is related to the duplicated gene SAM0232/SAM7441. One of the team’s projects is the identification and structural characterization of this molecule, and the present internship took place in this context. As such, the main objective was to construct a S. ambofaciens mutant strain with both genes SAM0232 and SAM7441 deleted, in order to identify and isolate the signaling molecule through comparative chromatography. An alternative method based on the heterologous expression of SAM0232 was also undertaken, using both Escherichia coli and S. coelicolor strains as expression hosts. However, it has not yet been possible to reproducibly identify the signaling molecule through this approach. A second part of this project dealt with the study of the suspected role the genes SAM0157 and SAM0158 play in the diazo group formation, a characteristic feature of the kinamycin molecule. The aim was to build mutants of these genes to validate this function by phenotypical and chromatographic analysis.
Document type :
Master thesis
File URL :
http://docnum.univ-lorraine.fr/ulprive/BUS_M_2017_BOUTILLIAT_ALEXIS.pdf
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https://hal.univ-lorraine.fr/hal-02337422
Contributor : Thèses Ul <>
Submitted on : Tuesday, October 29, 2019 - 2:21:35 PM
Last modification on : Wednesday, October 30, 2019 - 1:37:31 AM

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Alexis Boutilliat. Production de la Kinamycine chez Streptomyces ambofaciens : caractérisation de la molécule signal régulatrice et de la voie de la biosynthèse.. Microbiologie et Parasitologie. 2017. ⟨hal-02337422⟩

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