Recombinant pediocin in L actococcus lactis : increased production by propeptide fusion and improved potency by co‐production with PedC - Université de Lorraine Accéder directement au contenu
Article Dans Une Revue Microbial Biotechnology Année : 2016

Recombinant pediocin in L actococcus lactis : increased production by propeptide fusion and improved potency by co‐production with PedC

Alexandre Back
  • Fonction : Auteur
  • PersonId : 776925
  • IdRef : 184094488
Emmanuel Rondags
  • Fonction : Auteur
  • PersonId : 767695
  • IdRef : 156236435
Romain Kapel

Résumé

We describe the impact of two propeptides and PedC on the production yield and the potency of recombinant pediocins produced in Lactococcus lactis. On the one hand, the sequences encoding the propeptides SD or LEISSTCDA were inserted between the sequence encoding the signal peptide of Usp45 and the structural gene of the mature pediocin PA-1. On the other hand, the putative thiol-disulfide oxidoreductase PedC was coexpressed with pediocin. The concentration of recombinant pediocins produced in supernatants was determined by enzyme-linked immunosorbent assay. The potency of recombinant pediocins was investigated by measuring the minimal inhibitory concentration by agar well diffusion assay. The results show that propeptides SD or LEISSTCDA lead to an improved secretion of recombinant pediocins with apparently no effect on the antibacterial potency and that PedC increases the potency of recombinant pediocin. To our knowledge, this study reveals for the first time that pediocin tolerates fusions at the N-terminal end. Furthermore, it reveals that only expressing the pediocin structural gene in a heterologous host is not sufficient to get an optimal potency and requires the accessory protein PedC. In addition, it can be speculated that PedC catalyses the correct formation of disulfide bonds in pediocin.
Fichier principal
Vignette du fichier
Back-2016-Recombinant-pediocin-in------------.pdf (1.68 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03183303 , version 1 (27-03-2021)

Licence

Paternité

Identifiants

Citer

Alexandre Back, Frédéric Borges, Cecile Mangavel, Cédric Paris, Emmanuel Rondags, et al.. Recombinant pediocin in L actococcus lactis : increased production by propeptide fusion and improved potency by co‐production with PedC. Microbial Biotechnology, 2016, 9 (4), pp.466-477. ⟨10.1111/1751-7915.12285⟩. ⟨hal-03183303⟩
113 Consultations
64 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More