Skip to Main content Skip to Navigation
Journal articles

In situ cell differentiation monitoring of Catharanthus roseus suspension culture processes by NIR spectroscopy

Abstract : Plant suspension culture is attracting interest as a promising platform to produce biological medicines due to the absence of virus, prions or DNA related to mammals during the production process. However, the heterogenic plant cell proliferation nature is particularly challenging for establishing industrial processes based on innovative approaches currently used, particularly in the animal cell culture industry. In this context, while Process Analytical Technology (PAT) tools have been used to monitor classical parameters such as biomass dry weight, its use in cells heterogeneity has received limited attention. Therefore, the feasibility of in situ monitoring of cell differentiation in plant cell suspensions employing NIR spectroscopy and chemometrics was investigated. Off-line measurements of cell heterogeneity in term of cell differentiation and in-line NIR spectra captured in 3 L bioreactor cultures were employed to generate calibration models. Then models were tested to estimate the population distribution of parenchyma, collenchyma and sclerenchyma cells during Catharanthus roseus suspension cultures. Results have proven in situ NIR spectroscopy as a capable PAT tool to monitor differentiated cells accurately and in real-time. These results are the starting point to follow-up PAT systems so that plant cell culture heterogeneity may be better understood and controlled in biopharmaceutical plant cell cultures.
Complete list of metadatas

Cited literature [17 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/hal-02366817
Contributor : Annie Marc <>
Submitted on : Wednesday, September 2, 2020 - 11:21:16 AM
Last modification on : Wednesday, September 2, 2020 - 12:18:50 PM

File

In-situ cell differentiation m...
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Daniel Zavala-Ortiz, Bruno Ebel, Emmanuel Guedon, Annie Marc, Dulce Barradas-Dermitz, et al.. In situ cell differentiation monitoring of Catharanthus roseus suspension culture processes by NIR spectroscopy. Bioprocess and Biosystems Engineering, Springer Verlag, 2020, 43, pp.747-752. ⟨10.1007/s00449-019-02255-x⟩. ⟨hal-02366817⟩

Share

Metrics

Record views

334

Files downloads

58