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Journal Articles Biophysical Journal Year : 2016

Mechanical Criterion for the Rupture of a Cell Membrane under Compression

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Abstract

We investigate the mechanical conditions leading to the rupture of the plasma membrane of an endothelial cell subjected to a local, compressive force. Membrane rupture is induced by tilted microindentation, a technique used to perform mechanical measurements on adherent cells. In this technique, the applied force can be deduced from the measured horizontal displacement of a microindenter’s tip, as imaged with an inverted microscope and without the need for optical sensors to measure the microindenter’s deflection. We show that plasma membrane rupture of endothelial cells occurs at a well-defined value of the applied compressive stress. As a point of reference, we use numerical simulations to estimate the magnitude of the compressive stresses exerted on endothelial cells during the deployment of a stent.

Dates and versions

hal-02899524 , version 1 (15-07-2020)

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Cite

David Gonzalez-Rodriguez, Lionel Guillou, François Cornat, Julie Lafaurie-Janvore, Avin Babataheri, et al.. Mechanical Criterion for the Rupture of a Cell Membrane under Compression. Biophysical Journal, 2016, 111 (12), pp.2711-2721. ⟨10.1016/j.bpj.2016.11.001⟩. ⟨hal-02899524⟩
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