Skip to Main content Skip to Navigation
Journal articles

HydraPsiSeq: a method for systematic and quantitative mapping of pseudouridines in RNA

Abstract : Developing methods for accurate detection of RNA modifications remains a major challenge in epitranscriptomics. Next-generation sequencing-based mapping approaches have recently emerged but, often, they are not quantitative and lack specificity. Pseudouridine (), produced by uridine isomerization, is one of the most abundant RNA modification. mapping classically involves derivatization with soluble carbodiimide (CMCT), which is prone to variation making this approach only semi-quantitative. Here, we developed 'HydraPsiSeq', a novel quantitative mapping technique relying on specific protection from hydrazine/aniline cleavage. HydraPsiSeq is quantitative because the obtained signal directly reflects pseudouridine level. Furthermore, normalization to natural unmodified RNA and/or to synthetic in vitro transcripts allows absolute measurements of modification levels. HydraPsiSeq requires minute amounts of RNA (as low as 10-50 ng), making it compatible with high-throughput profiling of diverse biological and clinical samples. Exploring the potential of HydraPsiSeq, we profiled human rRNAs, revealing strong variations in pseudouridylation levels at ∼20-25 positions out of total 104 sites. We also observed the dynamics of rRNA pseudouridylation throughout chondrogenic differentiation of human bone marrow stem cells. In conclusion, HydraPsiSeq is a robust approach for the systematic mapping and accurate quantification of pseudouridines in RNAs with applications in disease, aging, development, differentiation and/or stress response.
Complete list of metadata
Contributor : JULIE GONNET Connect in order to contact the contributor
Submitted on : Thursday, November 26, 2020 - 1:28:07 PM
Last modification on : Thursday, May 19, 2022 - 1:52:03 PM
Long-term archiving on: : Saturday, February 27, 2021 - 7:10:26 PM


Files produced by the author(s)



Virginie Marchand, Florian Pichot, Paul Neybecker, Lilia Ayadi, Valérie Bourguignon-Igel, et al.. HydraPsiSeq: a method for systematic and quantitative mapping of pseudouridines in RNA. Nucleic Acids Research, Oxford University Press, 2020, 48 (19), pp.e110. ⟨10.1093/nar/gkaa769⟩. ⟨hal-02957799⟩



Record views


Files downloads