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A fast, efficient and easy to implement method to purify bacterial pili from Lacticaseibacillus rhamnosus GG based on multimodal chromatography

Abstract : Pili are polymeric proteins located at the cell surface of bacteria. These filamentous proteins play a pivotal role in bacterial adhesion with the surrounding environment. They are found both in Gram-negative and Gram-positive bacteria but differ in their structural organization. Purifying these high molecular weight proteins is challenging and has certainly slowed down
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https://hal.univ-lorraine.fr/hal-03059305
Contributor : Claire Gaiani <>
Submitted on : Monday, January 4, 2021 - 9:28:58 AM
Last modification on : Monday, January 11, 2021 - 10:48:07 AM

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Raphael dos Santos Morais, Sofiane El-Kirat-Chatel, Jennifer Burgain, Blandine Simard, Sarah Barrau, et al.. A fast, efficient and easy to implement method to purify bacterial pili from Lacticaseibacillus rhamnosus GG based on multimodal chromatography. Frontiers in Microbiology, Frontiers Media, 2020, 11, pp.609880. ⟨10.3389/fmicb.2020.609880⟩. ⟨hal-03059305⟩

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