One-year follow-up of microbial diversity in bioaerosols emitted in a waste sorting plant in France - Université de Lorraine
Journal Articles Waste Management Year : 2021

One-year follow-up of microbial diversity in bioaerosols emitted in a waste sorting plant in France

Abstract

Bioaerosols emitted in waste sorting plants (WSP) can induce some adverse health effects on the workers such as rhinitis, asthma and hypersensitivity pneumonitis. The composition of these bioaerosols is scarcely known and most of the time assessed using culture-dependent methods. Due to the well-known limitations of cultural methods, these biodiversity measurements underestimate the actual microbial taxon richness. The aim of the study was to assess the airborne microbial biodiversity by using a sequencing method in a French waste sorting plant (WSP) for one year and to investigate the main factors of variability of this biodiversity. Static sampling was performed in five areas in the plant and compared to an indoor reference (IR), using closed-face cassettes (10 L.min−1) with polycarbonate membranes, every month for one year. Environmental data was measured (temperature, relative humidity). After DNA extraction, microbial biodiversity was assessed by means of sequencing. Bacterial genera Staphylococcus, Streptococcus, Prevotella, Lactococcus, Lactobacillus, Pseudomonas and fungal genera Wallemia, Cladosporium, Debaryomyces, Penicillium, Alternaria were the most predominant airborne microorganisms. Microbial biodiversity was different in the plant compared to the IR and seemed to be influenced by the season.
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hal-03070350 , version 1 (16-12-2022)

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Jodelle Degois, Xavier Simon, Frédéric Clerc, Cyril Bontemps, Pierre Leblond, et al.. One-year follow-up of microbial diversity in bioaerosols emitted in a waste sorting plant in France. Waste Management, 2021, 120, pp.257-268. ⟨10.1016/j.wasman.2020.11.036⟩. ⟨hal-03070350⟩
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