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Curcumin Efficacy in a Serum/glucose Deprivation-induced Neuronal PC12 Injury Model

Abstract : Background: Glucose/serum deprivation (GSD), has been used for understanding molecular mechanisms of neuronal damage during ischemia. It has been suggested that curcumin may improve neurodegenerative diseases. Aim: In this study, the protective effects of curcumin and its underlying mechanisms were investigated in PC12 cells upon GSD-induced stress. Methods: PC12 cells were cultured in DMEM overnight and then incubated in GSD condition for either 6 or 12h. GSD-treated cells were pretreated with various concentrations of curcumin (10, 20, and 40 M) for 5h. The cell viability, apoptosis, reactive oxygen species (ROS) level, oxidative stress, expression of apoptosis-related genes, and IL-6 were determined. Results: Curcumin increased cell viability and caused an anti-apoptotic effect in PC12 cells exposed for 12h to GSD . Curcumin also increased antioxidant enzyme expression, suppressed lipid peroxidation, and decreased interleukin-6 secretion in PC12 cells subjected to GSD. In addition, pretreatment with curcumin down-regulated pro-apoptotic (Bax), and up-regulated antiapoptotic (Bcl2) mediators. Conclusion: Curcumin mitigates many of the adverse effects of ischemia, and therefore, should be considered as an adjunct therapy in ischemic patients.
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Submitted on : Friday, June 25, 2021 - 11:04:47 AM
Last modification on : Wednesday, September 15, 2021 - 4:29:17 PM

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Tahereh Farkhondeh, Milad Ashrafizadeh, Mohsen Azimi-Nezhad, Fariborz Samini, Micheal Aschenr, et al.. Curcumin Efficacy in a Serum/glucose Deprivation-induced Neuronal PC12 Injury Model. Current Molecular Pharmacology, Bentham Science, 2021, 14, ⟨10.2174/1874467214666210203211312⟩. ⟨hal-03270873⟩



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